524001

antibody from BioLegend

Targeting: MIF GIF, GLIF

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

524001

Provider

BioLegend

Proper citation

BioLegend Cat#524001, RRID:AB_2562323

Product name

Purified anti-mouse/rat/human MIF

Antibody type

Monoclonal

Antigen

Purified recombinant mouse MIF (AA: Pro2-Ala115) expressed in E. coli (Cat# 599504).

Reactivity

Human, Mouse, Rat

Host

Goat

Conjugate

Unconjugated

Antibody clone number

Poly5240

Vial size

200 µl

Storage

-20°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Western blot analysis of cell lysates from HeLa (lane 1), U937 (lane 2), Raw264.7 (lane 3), and rat brain (lane 4). Purified anti-MIF antibody (clone Poly5240) was used and was followed by HRP anti-goat IgG antibody. GAPDH (clone Poly6314) was used as a loading control.

Conjugate

Unconjugated