Antibody data
- Product number
- PA5-27343
- Provider
- Invitrogen Antibodies
- Product name
- Anti-MIF Polyclonal Antibody
- Provider product page
- Invitrogen Antibodies - PA5-27343
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Molt-4, mouse brain. Predicted reactivity: Mouse (89%), Rat (90%), Pig (94%), Sheep (91%), Rhesus Monkey (100%), Bovine (91%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis MIF was performed by loading 30 µg of HEK293T whole cell lysate per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat dry milk in TBST for 1 hour at room temperature. The membrane was probed with a MIF polyclonal antibody (Product # PA5-27343) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:40,000 for 1 hour at room temperature. Detection was performed using ECL substrate. Data courtesy of the Innovators Program.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MIF using 30 µg of MOLT4 lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with a MIF polyclonal antibody (Product # PA5-27343) at a dilution of 1:1000.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MIF using 30 µg of NIH-3T3 lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with a MIF polyclonal antibody (Product # PA5-27343) at a dilution of 1:1000.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MIF was performed by separating 50 µg of various tissue extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a MIF Polyclonal Antibody (Product # PA5-27343) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA detection of MIF using a MIF polyclonal antibody (Product # PA5-27343) for capture at a concentration of 5 µg/mL, and an alternate MIF antibody for detection at a concentration of 1.5 µg/mL.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 14-3-3 zeta (green) HEK293T cells. Cells fixed with 4% formaldehyde fixed were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with a 14-3-3 zeta ppolyclonal antibody (Product # PA5-27317) at a dilution of 1:100 overnight at 4°C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with fluorophore-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI. Images were taken on a Leica DM1000 microscope at 40X magnification. Data courtesy of the Innovators Program.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MIF in methanol-fixed HeLa cells using a MIF polyclonal antibody (Product # PA5-27343) at a 1:200 dilution.
Supportive data in Antibodypedia
Data presented on provider website
