PA5-59516

antibody from Invitrogen Antibodies

Targeting: CYP51A1 CP51, CYP51, CYPL1, LDM, P450-14DM, P450L1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-59516

Provider

Invitrogen Antibodies

Product name

CYP51A1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CYP51A1 in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a CYP51A1 Polyclonal Antibody (Product # PA5-59516). Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of CYP51A1 in human cell line A-431 shows positivity in endoplasmic reticulum. Samples were probed using a CYP51A1 Polyclonal Antibody (Product # PA5-59516).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CYP51A1 in human colon using CYP51A1 Polyclonal Antibody (Product # PA5-59516).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CYP51A1 in human liver using CYP51A1 Polyclonal Antibody (Product # PA5-59516).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CYP51A1 in human cerebral cortex using CYP51A1 Polyclonal Antibody (Product # PA5-59516).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CYP51A1 in human cerebral cortex, colon, liver and testis using CYP51A1 Polyclonal Antibody (Product # PA5-59516) (A) shows similar protein distribution across tissues to an independent CYP51A1 Polyclonal Antibody (B).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CYP51A1 in human testis using CYP51A1 Polyclonal Antibody (Product # PA5-59516) shows weak to moderate cytoplasmic positivity in Leydig cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CYP51A1 in human skeletal muscle using CYP51A1 Polyclonal Antibody (Product # PA5-59516) shows no cytoplasmic positivity in myocytes.