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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- 34-3700 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Ephrin A4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
Submitted references Evaluation of Eph receptor and ephrin expression within the human cornea and limbus.
Genome-wide analysis of promoter methylation associated with gene expression profile in pancreatic adenocarcinoma.
Hogerheyde TA, Stephenson SA, Harkin DG, Bray LJ, Madden PW, Woolf MI, Richardson NA
Experimental eye research 2013 Feb;107:110-20
Experimental eye research 2013 Feb;107:110-20
Genome-wide analysis of promoter methylation associated with gene expression profile in pancreatic adenocarcinoma.
Vincent A, Omura N, Hong SM, Jaffe A, Eshleman J, Goggins M
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Jul 1;17(13):4341-54
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Jul 1;17(13):4341-54
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Ephrin A4 was performed using 70% confluent log phase Jurkat cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Ephrin A4 Rabbit Polyclonal Antibody (Product # 34-3700) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
