F47908

antibody from NSJ Bioreagents

Targeting: SOX2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F47908

Provider

NSJ Bioreagents

Product name

SOX-2 Antibody

Antibody type

Polyclonal

Antigen

A portion of amino acids 89-119 from the human protein was used as the immunogen for this SOX-2 antibody.

Description

Purified antibody

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

80 µl, 400 µl

Storage

Aliquot the SOX-2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of SOX-2 antibody and 293 lysate

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of SOX-2 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (2).

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescence analysis of SOX-2 antibody and HeLa cells. Primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC analysis of FFPE human lung carcinoma tissue stained with SOX-2 antibody

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometric analysis of NCI-H292 cells using SOX-2 antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.