NB110-37235

antibody from Novus Biologicals

Targeting: SOX2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB110-37235

Provider

Novus Biologicals

Proper citation

Novus Cat#NB110-37235, RRID:AB_792070

Product name

Rabbit Polyclonal SOX2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat, Canine, Sheep

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

1.0 mg/ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

α-Mangostin attenuates stemness and enhances cisplatin-induced cell death in cervical cancer stem-like cells through induction of mitochondrial-mediated apoptosis.

Chien HJ, Ying TH, Hsieh SC, Lin CL, Yu YL, Kao SH, Hsieh YH
Journal of cellular physiology 2020 Jul;235(7-8):5590-5601

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Chemotherapy-induced S100A10 recruits KDM6A to facilitate OCT4-mediated breast cancer stemness.

Lu H, Xie Y, Tran L, Lan J, Yang Y, Murugan NL, Wang R, Wang YJ, Semenza GL
The Journal of clinical investigation 2020 Jul 27;

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Oligodendroglioma with Neuronal Differentiation in Two Boxer Dogs.

Cornax I, Pluhar GE, Clark HB, O'Sullivan MG
Journal of comparative pathology 2019 Oct;172:11-16

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Microfluidic reprogramming to pluripotency of human somatic cells.

Gagliano O, Luni C, Qin W, Bertin E, Torchio E, Galvanin S, Urciuolo A, Elvassore N
Nature protocols 2019 Mar;14(3):722-737

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Macrophage-Derived Slit3 Controls Cell Migration and Axon Pathfinding in the Peripheral Nerve Bridge.

Dun XP, Carr L, Woodley PK, Barry RW, Drake LK, Mindos T, Roberts SL, Lloyd AC, Parkinson DB
Cell reports 2019 Feb 5;26(6):1458-1472.e4

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Bone marrow-derived fibrocytes promote stem cell-like properties of lung cancer cells.

Saijo A, Goto H, Nakano M, Mitsuhashi A, Aono Y, Hanibuchi M, Ogawa H, Uehara H, Kondo K, Nishioka Y
Cancer letters 2018 May 1;421:17-27

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Sox2 expression in Schwann cells inhibits myelination in vivo and induces influx of macrophages to the nerve.

Roberts SL, Dun XP, Doddrell RDS, Mindos T, Drake LK, Onaitis MW, Florio F, Quattrini A, Lloyd AC, D'Antonio M, Parkinson DB
Development (Cambridge, England) 2017 Sep 1;144(17):3114-3125

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Chemotherapy-Induced Ca²⁺ Release Stimulates Breast Cancer Stem Cell Enrichment.

Lu H, Chen I, Shimoda LA, Park Y, Zhang C, Tran L, Zhang H, Semenza GL
Cell reports 2017 Feb 21;18(8):1946-1957

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SIRT1-mediated transcriptional regulation of SOX2 is important for self-renewal of liver cancer stem cells.

Liu L, Liu C, Zhang Q, Shen J, Zhang H, Shan J, Duan G, Guo D, Chen X, Cheng J, Xu Y, Yang Z, Yao C, Lai M, Qian C
Hepatology (Baltimore, Md.) 2016 Sep;64(3):814-27

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Hypoxia-inducible factors regulate pluripotency factor expression by ZNF217- and ALKBH5-mediated modulation of RNA methylation in breast cancer cells.

Zhang C, Zhi WI, Lu H, Samanta D, Chen I, Gabrielson E, Semenza GL
Oncotarget 2016 Oct 4;7(40):64527-64542

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Neural stem cells from mouse strain Thy1 YFP-16 are a valuable tool to monitor and evaluate neuronal differentiation and morphology.

Alić I, Kosi N, Kapuralin K, Gorup D, Gajović S, Pochet R, Mitrečić D
Neuroscience letters 2016 Nov 10;634:32-41

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High-efficiency cellular reprogramming with microfluidics.

Luni C, Giulitti S, Serena E, Ferrari L, Zambon A, Gagliano O, Giobbe GG, Michielin F, Knöbel S, Bosio A, Elvassore N
Nature methods 2016 May;13(5):446-52

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Predicting the molecular role of MEIS1 in esophageal squamous cell carcinoma.

Rad A, Farshchian M, Forghanifard MM, Matin MM, Bahrami AR, Geerts D, A'rabi A, Memar B, Abbaszadegan MR
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Feb;37(2):1715-25

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Plasminogen activator inhibitor-1 as regulator of tumor-initiating cell properties in head and neck cancers.

Lee YC, Yu CC, Lan C, Lee CH, Lee HT, Kuo YL, Wang PH, Chang WW
Head & neck 2016 Apr;38 Suppl 1:E895-904

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Chemotherapy triggers HIF-1-dependent glutathione synthesis and copper chelation that induces the breast cancer stem cell phenotype.

Lu H, Samanta D, Xiang L, Zhang H, Hu H, Chen I, Bullen JW, Semenza GL
Proceedings of the National Academy of Sciences of the United States of America 2015 Aug 18;112(33):E4600-9

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Pluripotent state induction in mouse embryonic fibroblast using mRNAs of reprogramming factors.

El-Sayed AK, Zhang Z, Zhang L, Liu Z, Abbott LC, Zhang Y, Li B
International journal of molecular sciences 2014 Nov 27;15(12):21840-64

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Efficient germ-line transmission obtained with transgene-free induced pluripotent stem cells.

Wu S, Wu Y, Zhang X, Capecchi MR
Proceedings of the National Academy of Sciences of the United States of America 2014 Jul 22;111(29):10678-83

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Beneficial actions of the anti-inflammatory dimethyl fumarate in glioblastomas.

Ghods AJ, Glick R, Braun D, Feinstein D
Surgical neurology international 2013;4:160

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Wnt/Rspondin/β-catenin signals control axonal sorting and lineage progression in Schwann cell development.

Grigoryan T, Stein S, Qi J, Wende H, Garratt AN, Nave KA, Birchmeier C, Birchmeier W
Proceedings of the National Academy of Sciences of the United States of America 2013 Nov 5;110(45):18174-9

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Connexin 43 reverses malignant phenotypes of glioma stem cells by modulating E-cadherin.

Yu SC, Xiao HL, Jiang XF, Wang QL, Li Y, Yang XJ, Ping YF, Duan JJ, Jiang JY, Ye XZ, Xu SL, Xin YH, Yao XH, Chen JH, Chu WH, Sun W, Wang B, Wang JM, Zhang X, Bian XW
Stem cells (Dayton, Ohio) 2012 Feb;30(2):108-20

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Signaling through BMP receptors promotes respiratory identity in the foregut via repression of Sox2.

Domyan ET, Ferretti E, Throckmorton K, Mishina Y, Nicolis SK, Sun X
Development (Cambridge, England) 2011 Mar;138(5):971-81

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Sox2 protein expression is an independent poor prognostic indicator in stage I lung adenocarcinoma.

Sholl LM, Barletta JA, Yeap BY, Chirieac LR, Hornick JL
The American journal of surgical pathology 2010 Aug;34(8):1193-8

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SOX2 Antibody [NB110-37235] - Detection of SOX2 in mouse brain lysate using NB110-37235 at 0.5 ug/mL.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SOX2 Antibody [NB110-37235] - Expression of pluripotency factor Sox2 in human head and neck cancer cell lines FaDu and HN5. WB image submitted by a verified customer review.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SOX2 Antibody [NB110-37235] - MDA-MB-231 subclones transfected with NTC or HIF-1 alpha shRNA vector were treated with vehicle (V) or paclitaxel (P) and immunoblot assay was performed. WB image submitted by a verified customer review.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SOX2 Antibody [NB110-37235] - Staining of human uterus, endometrial glands.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: SOX2 Antibody [NB110-37235] - An intracellular stain was performed on A549 with NB110-37235 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylgiht 550.