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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- AM11050PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM11050PU-N, RRID:AB_1771240
- Product name
- anti SOX2
- Antibody type
- Monoclonal
- Antigen
- SOX2 recombinant protein.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 57CT23.3.4
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- LEFT: Western blot analysis of anti-SOX2 Monoclonal Antibody (Cat.#AM11050PU-N) by SOX2 recombinant protein. SOX2(arrow) was detected using the purified Mab. RIGHT: Western blot analysis of SOX2 (arrow) using mouse monoclonal SOX2 antibody (Cat.#AM11050PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2). Origene Technologies.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of SOX2 Monoclonal antibody (Cat.#AM11050PU-N) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 Monoclonal Antibody (Cat.#AM11050PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of SOX2 Monoclonal antibody with paraffin-embedded Human lung carcinoma tissue. 0.025 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule). PE emits orange fluorescence.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NCI-H460 cells using SOX2 Monoclonal Antibody (bottom histogram) compared to a Negative Control cell (top histogram). PE-conjugated Goat-anti-Mouse secondary antibodies were used for the analysis.
