- GTX627404 - GeneTex SOX2 antibody

Submitted references Non-Canonical Regulation of Type I Collagen through Promoter Binding of SOX2 and Its Contribution to Ameliorating Pulmonary Fibrosis by Butylidenephthalide.

Chuang HM, Ho LI, Huang MH, Huang KL, Chiou TW, Lin SZ, Su HL, Harn HJ
International journal of molecular sciences 2018 Oct 4;19(10)

Thyroid hormone negatively regulates tumorigenesis through suppression of BC200.

Lin YH, Wu MH, Huang YH, Yeh CT, Chi HC, Tsai CY, Chuang WY, Yu CJ, Chung IH, Chen CY, Lin KH
Endocrine-related cancer 2018 Dec 1;25(12):967-979

Stress stimuli induce cancer-stemness gene expression via Sp1 activation leading to therapeutic resistance in glioblastoma.

Chang KY, Huang CT, Hsu TI, Hsu CC, Liu JJ, Chuang CK, Hung JJ, Chang WC, Tsai KK, Chuang JY
Biochemical and biophysical research communications 2017 Nov 4;493(1):14-19

DDX3 Represses Stemness by Epigenetically Modulating Tumor-suppressive miRNAs in Hepatocellular Carcinoma.

Li HK, Mai RT, Huang HD, Chou CH, Chang YA, Chang YW, You LR, Chen CM, Lee YH
Scientific reports 2016 Jun 27;6:28637

The reciprocal regulation loop of Notch2 pathway and miR-23b in controlling gastric carcinogenesis.

Huang TT, Ping YH, Wang AM, Ke CC, Fang WL, Huang KH, Lee HC, Chi CW, Yeh TS
Oncotarget 2015 Jul 20;6(20):18012-26

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOX2 antibody [GT1876] detects SOX2 protein by Western blot analysis.A. 30 £gg human ESC whole cell lysate/extract10 % SDS-PAGESOX2 antibody [GT1876] (GTX627404) dilution: 1:5000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOX2 antibody [GT1876] detects SOX2 protein by Western blot analysis.A. 30 ?g zebrafish eye lysate/extract10 % SDS-PAGESOX2 antibody [GT1876] (GTX627404) dilution: 1:500

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG D: SK-N-SH 10% SDS PAGE GTX627404 diluted at 1:5000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) NT2D1 whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody [GT1876] (GTX627404) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Whole cell extract (30 ?g) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody [GT1876] (GTX627404) diluted at 1:1000 and competitor's antibody (sc-17320) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.*The competitor is not affiliated with GeneTex and does not endorse this product.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) NT2D1 whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody [GT1876] (GTX627404) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOX2 antibody [GT1876] immunoprecipitates SOX2 protein in IP experiments.IP samples: NT2D1 nuclear extractA. 50 ?g NT2D1 nuclear extractB. Control with 4 ?g of preimmune Rabbit IgGC. Immunoprecipitation of SOX2 protein by 4 ?g SOX2 antibody [GT1876] (GTX627404)10 % SDS-PAGEThe immunoprecipitated SOX2 protein was detected by SOX2 antibody [GT1876] (GTX627404) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Validation comment: IP

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 £gg of SOX2 antibody [GT1876] (GTX627404) or SOX2 antibody [N1C3] (GTX101057).Western blot analysis was performed using SOX2 antibody [GT1876] (GTX627404).EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sox2 antibodies detects Sox2 proteins on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Green: GFAP antibody (GTX100850) diluted at 1:500. Red: Sox2 antibody [GT1876] (GTX627404) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOX2 antibody [GT1876] detects SOX2 protein by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina.Green: SOX2 protein stained by SOX2 antibody [GT1876] (GTX627404) diluted at 1:250. Red: PKC alpha protein stained by PKC alpha antibody [GT1876] (GTX130453) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOX2 antibody [GT1876] detects SOX2 protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: SOX2 antibody [GT1876] dilution: 1:50

GTX627404

Antibody data