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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- 15264-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Adenylosuccinate lyase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAAGGDHGS PDSYRSPLAS RYASPEMCFV FSDRYKFRTW RQLWLWLAEA EQTLGLPITD EQIQEMKSNL ENIDFKMAAE EEKRLRHDVM AHVHTFGHCC PKAAGIIHLG ATSCYVGDNT DLIILRNALD LLLPKLARVI SRLADFAKER ASLPTLGFTH FQPAQLTTVG KRCCLWIQDL CMDLQNLKRV RDDLRFRGVK GTTGTQASFL QLFEGDDHKV EQLDKMVTEK AGFKRAFIIT GQTYTRKVDI EVLSVLASLG ASVHKICTDI RLLANLKEME EPFEKQQIGS SAMPYKRNPM RSERCCSLAR HLMTLVMDPL QTASVQWFER TLDDSANRRI CLAEAFLTAD TILNT (1-354 aa encoded by BC000253)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.22 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 15264-1-AP (Adenylosuccinate lyase antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 15264-1-AP (Adenylosuccinate lyase antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using ADSL antibody 15264-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer using 15264-1-AP (Adenylosuccinate lyase antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer using 15264-1-AP (Adenylosuccinate lyase antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with 0.2ug Adenylosuccinate lyase antibody (15264-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.
