MA5-41039

antibody from Invitrogen Antibodies

Targeting: ADSL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-41039

Provider

Invitrogen Antibodies

Product name

ADSL Recombinant Rabbit Monoclonal Antibody (007)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

007

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ADSL Recombinant Rabbit Monoclonal Antibody (007) (Product # MA5-41039) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: Jurkat Whole Cell Lysate, Lane C: Hela Whole Cell Lysate, Lane D: C6 Whole Cell Lysate, Lane E: Raw264.7 Whole Cell Lysate, Lane F: NIH-3T3 Whole Cell Lysate, Lane G: HCT-116 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 55 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of ADSL in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with ADSL Recombinant Rabbit Monoclonal Antibody (007) (Product # MA5-41039, 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of ADSL in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with ADSL Recombinant Rabbit Monoclonal Antibody (007) (Product # MA5-41039, 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of ADSL in Lane A: 0.5 mg HepG2 Whole Cell Lysate. Samples were treated with 15 μl of 50 % Protein G agarose, incubated with monoclonal antibody (Product # MA5-41039) with a dilution of 1:200 , followed by Clean-Blot HRP Detection reagent using a dilution of 1:1,000. Assay was performed under reducing conditions. Predicted band size: 50 kDa, Observed band size: 50 kDa .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ADSL Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 2 µL with ADSL Recombinant Rabbit Monoclonal Antibody (007) (Product # MA5-41039) and 15 µL of 50 % Protein G agarose. Primary antibody: ADSL Recombinant Rabbit Monoclonal Antibody (007), at 1:200 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1,000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 50 kDa.