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Learn200-301-H54
antibody from Rockland Immunochemicals, Inc.
Targeting: AR
AIS, DHTR, HUMARA, NR3C4, SBMA, SMAX1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 200-301-H54 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-301-H54, RRID:AB_2611277
- Product name
- Anti-Androgen Receptor (MOUSE) Monoclonal Antibody - 200-301-H54
- Antibody type
- Monoclonal
- Vial size
- 100 µl
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of Mouse Anti-Androgen Receptor antibody. Lane A: LNCaP untreated cells. Lane B: LNCaP cells treated with 100ng IGF-1 for 4hrs. Lane C: LNCaP cells incubated with 20µm LY294002 for 30min prior to treatment with 100ng IGF-1 for 4hrs. Load: 30 µg per lane. Primary antibody: Androgen Receptor antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800 mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~110 kDa for Androgen Receptor antibody. Other band(s): none.
- Validation comment
- Western Blot
