Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- ARG63608 - Provider product page
- Provider
- Arigo
- Product name
- anti-Androgen Receptor antibody - ChIP-Grade
- Antibody type
- Polyclonal
- Antigen
- EVQLGLGRVYPRPPSC
- Description
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: Human Brain lysate (35 µg protein in RIPA buffer) stained with ARG63608 anti-Androgen Receptor antibody - ChIP-Grade at 0.3 µg/ml dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin embedded Human Prostate. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63608 anti-Androgen Receptor antibody - ChIP-Grade at 2 µg/ml dilution followed by AP-staining.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- ChIP: 2 µg ARG63608 anti-Androgen Receptor antibody - ChIP-Grade with 1 µg DHT-treated HEC50 chromatin using the using the Chromatrap® spin column sonication kit (Protein G) measuring FKBP5 enrichment.