Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-16750 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Androgen Receptor Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA5-16750 targets Androgen Receptor in IF, IHC (P), IP, and WB applications and shows reactivity with human, mouse, and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 500 µL
- Concentration
- 0.2 mg/mL
- Storage
- 4° C
Submitted references Estradiol alleviates acinar cell apoptosis and chronic pancreatitis in male Wistar Bonn/Kobori rats.
Nakamura S, Yamada T, Hashimoto T, Takahashi S, Sogawa M, Ohara H, Nakazawa T, Sano H, Kuno A, Joh T, Nomura T, Arakawa T, Itoh M
Pancreas 2003 Apr;26(3):e59-66
Pancreas 2003 Apr;26(3):e59-66
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Androgen Receptor was performed by loading 50 µg of LNCaP, MCF7, HeLa, and DU145 whole cell lysates, and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with StartingBlock T20 (Product # 37543) for 1 hour. The membrane was probed with an Androgen Receptor polyclonal antibody (Product # PA5-16750) at a dilution of 1:200 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with an HRP-conjugated goat anti-rabbit IgG secondary antibody (Product # 31460) at a dilution of 1:20,000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075). Images were acquired on a Thermo Scientific myECL Imager (Product # 62236).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Androgen Receptor polyclonal antibody (Product # PA5-16750) was used to detect immunoprecipitated Androgen Receptor. Immunoprecipitation of Androgen Receptor was performed on LNCaP cells. Antigen-antibody complexes were formed by incubating 250 µg of LNCaP whole cell lysate with 2 µg of a biotinylated Androgen Receptor monoclonal antibody (Product # MA5-13423) overnight on a rocking platform at 4C. The immune complexes were captured on 50 µL Streptavidin Agarose (Product # 20347), washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer (Product # 39000). Eluted sample and 20 µg of LNCaP whole cell lysate (loading control) were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with StartingBlock T20 (Product # 37543) for 1 hour. The membrane was probed with an Androgen Receptor polyclonal antibody (Product # PA5-16750) at a dilution of 1:200 overnight rotating at 4C, washed in TBST, and probed with an HRP-conjugated goat anti-rabbit IgG (Product # 31460) at a dilution of 1:20,000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075). Images were acquired on a Thermo Scientific myECL Imager (Product # 62236).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Androgen Receptor (green) in LNCaP (right panel) and negative control U2OS cells (left panel). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with an Androgen Receptor polyclonal antibody (Product # PA5-16750) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight-554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.