Antibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- NB100-56603 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56603, RRID:AB_837565
- Product name
- Mouse Monoclonal Androgen R/NR3C4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. In IGF-1 stimulated LNCaP cells (passage number 38), a ~110 kDa band was observed. Please see Lin et al. 2003 for additional details. The serine phosphorylation site recognized by this antibody has been alternatively referred to Ser213 (Lee and Chang, 2003) and Ser210 (Lin et al, 2003). Variations in denotation can arise from how the sequence is counted in various GenBank accession numbers. The site is denoted as Ser213 in GenBank Accession No. A39248, which was used to design the immunogen.
- Reactivity
- Human, Mouse, Rat, Canine, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Androgen receptor phosphorylation at serine 81 and serine 213 in castrate-resistant prostate cancer.
Estrogen receptor β expression and androgen receptor phosphorylation correlate with a poor clinical outcome in hormone-naive prostate cancer and are elevated in castration-resistant disease.
Androgen receptor serine 81 phosphorylation mediates chromatin binding and transcriptional activation.
Androgen regulated HN1 leads proteosomal degradation of androgen receptor (AR) and negatively influences AR mediated transactivation in prostate cells.
The CXCL12/CXCR4 axis promotes ligand-independent activation of the androgen receptor.
Differential transformation capacity of Src family kinases during the initiation of prostate cancer.
An AKT activity threshold regulates androgen-dependent and androgen-independent PSA expression in prostate cancer cell lines.
Akt blocks ligand binding and protects against expanded polyglutamine androgen receptor toxicity.
Hyaluronan stimulates transformation of androgen-independent prostate cancer.
Down-regulation of androgen receptor by 3,3'-diindolylmethane contributes to inhibition of cell proliferation and induction of apoptosis in both hormone-sensitive LNCaP and insensitive C4-2B prostate cancer cells.
Observer variation in immunohistochemical analysis of protein expression, time for a change?
A neuroactive steroid 5alpha-androstane-3alpha,17beta-diol regulates androgen receptor level in astrocytes.
Suppression versus induction of androgen receptor functions by the phosphatidylinositol 3-kinase/Akt pathway in prostate cancer LNCaP cells with different passage numbers.
McAllister MJ, McCall P, Dickson A, Underwood MA, Andersen D, Holmes E, Markert E, Leung HY, Edwards J
Prostate cancer and prostatic diseases 2020 May 1;
Prostate cancer and prostatic diseases 2020 May 1;
Estrogen receptor β expression and androgen receptor phosphorylation correlate with a poor clinical outcome in hormone-naive prostate cancer and are elevated in castration-resistant disease.
Zellweger T, Stürm S, Rey S, Zlobec I, Gsponer JR, Rentsch CA, Terracciano LM, Bachmann A, Bubendorf L, Ruiz C
Endocrine-related cancer 2013 Jun;20(3):403-13
Endocrine-related cancer 2013 Jun;20(3):403-13
Androgen receptor serine 81 phosphorylation mediates chromatin binding and transcriptional activation.
Chen S, Gulla S, Cai C, Balk SP
The Journal of biological chemistry 2012 Mar 9;287(11):8571-83
The Journal of biological chemistry 2012 Mar 9;287(11):8571-83
Androgen regulated HN1 leads proteosomal degradation of androgen receptor (AR) and negatively influences AR mediated transactivation in prostate cells.
Varisli L, Gonen-Korkmaz C, Syed HM, Bogurcu N, Debelec-Butuner B, Erbaykent-Tepedelen B, Korkmaz KS
Molecular and cellular endocrinology 2012 Mar 5;350(1):107-17
Molecular and cellular endocrinology 2012 Mar 5;350(1):107-17
The CXCL12/CXCR4 axis promotes ligand-independent activation of the androgen receptor.
Kasina S, Macoska JA
Molecular and cellular endocrinology 2012 Apr 4;351(2):249-63
Molecular and cellular endocrinology 2012 Apr 4;351(2):249-63
Differential transformation capacity of Src family kinases during the initiation of prostate cancer.
Cai H, Smith DA, Memarzadeh S, Lowell CA, Cooper JA, Witte ON
Proceedings of the National Academy of Sciences of the United States of America 2011 Apr 19;108(16):6579-84
Proceedings of the National Academy of Sciences of the United States of America 2011 Apr 19;108(16):6579-84
An AKT activity threshold regulates androgen-dependent and androgen-independent PSA expression in prostate cancer cell lines.
Paliouras M, Diamandis EP
Biological chemistry 2008 Jun;389(6):773-80
Biological chemistry 2008 Jun;389(6):773-80
Akt blocks ligand binding and protects against expanded polyglutamine androgen receptor toxicity.
Palazzolo I, Burnett BG, Young JE, Brenne PL, La Spada AR, Fischbeck KH, Howell BW, Pennuto M
Human molecular genetics 2007 Jul 1;16(13):1593-603
Human molecular genetics 2007 Jul 1;16(13):1593-603
Hyaluronan stimulates transformation of androgen-independent prostate cancer.
Lin SL, Chang D, Ying SY
Carcinogenesis 2007 Feb;28(2):310-20
Carcinogenesis 2007 Feb;28(2):310-20
Down-regulation of androgen receptor by 3,3'-diindolylmethane contributes to inhibition of cell proliferation and induction of apoptosis in both hormone-sensitive LNCaP and insensitive C4-2B prostate cancer cells.
Bhuiyan MM, Li Y, Banerjee S, Ahmed F, Wang Z, Ali S, Sarkar FH
Cancer research 2006 Oct 15;66(20):10064-72
Cancer research 2006 Oct 15;66(20):10064-72
Observer variation in immunohistochemical analysis of protein expression, time for a change?
Kirkegaard T, Edwards J, Tovey S, McGlynn LM, Krishna SN, Mukherjee R, Tam L, Munro AF, Dunne B, Bartlett JM
Histopathology 2006 Jun;48(7):787-94
Histopathology 2006 Jun;48(7):787-94
A neuroactive steroid 5alpha-androstane-3alpha,17beta-diol regulates androgen receptor level in astrocytes.
Agapova OA, Malone PE, Hernandez MR
Journal of neurochemistry 2006 Jul;98(2):355-63
Journal of neurochemistry 2006 Jul;98(2):355-63
Suppression versus induction of androgen receptor functions by the phosphatidylinositol 3-kinase/Akt pathway in prostate cancer LNCaP cells with different passage numbers.
Lin HK, Hu YC, Yang L, Altuwaijri S, Chen YT, Kang HY, Chang C
The Journal of biological chemistry 2003 Dec 19;278(51):50902-7
The Journal of biological chemistry 2003 Dec 19;278(51):50902-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) [NB100-56603] - Analysis using Azide Free version of NB100-56603. LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A) left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 mi