Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP1-83306 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-83306, RRID:AB_11024750
- Product name
- Rabbit Polyclonal NSDHL Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse, rat NSDHL antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Intercellular transfer of proteins as identified by stable isotope labeling of amino acids in cell culture.
Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E
Nature methods 2013 Apr;10(4):315-23
Nature methods 2013 Apr;10(4):315-23
Intercellular transfer of proteins as identified by stable isotope labeling of amino acids in cell culture.
Li M, Aliotta JM, Asara JM, Wu Q, Dooner MS, Tucker LD, Wells A, Quesenberry PJ, Ramratnam B
The Journal of biological chemistry 2010 Feb 26;285(9):6285-97
The Journal of biological chemistry 2010 Feb 26;285(9):6285-97
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NSDHL Antibody [NBP1-83306] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells). Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NSDHL Antibody [NBP1-83306] - Analysis using Anti-NSDHL antibody NBP1-83306 (A) shows similar pattern to independent antibody NBP1-83307 (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NSDHL Antibody [NBP1-83306] - Staining of human epididymis shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NSDHL Antibody [NBP1-83306] - Staining of human lymph node shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NSDHL Antibody [NBP1-83306] - Staining in human epididymis and lymph node tissues using anti-NSDHL antibody. Corresponding NSDHL RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NSDHL Antibody [NBP1-83306] - Staining of human cerebral cortex using Anti-NSDHL antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NSDHL Antibody [NBP1-83306] - Staining of human testis using Anti-NSDHL antibody.