Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [45]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX100064 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100064, RRID:AB_2036326
- Product name
- Bcl-2 antibody [N1N2], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Zebrafish
- Host
- Rabbit
Submitted references Enterococcus faecalis induces apoptosis and pyroptosis of human osteoblastic MG63 cells via the NLRP3 inflammasome.
Post-Injury Neuroprotective Effects of the Thalidomide Analog 3,6'-Dithiothalidomide on Traumatic Brain Injury.
Effects of ionizing radiation and HLY78 on the zebrafish embryonic developmental toxicity.
Effect of mild hypothermia on cerebral microcirculation in a murine cardiopulmonary resuscitation model.
Hepatorenal protective effects of taurine and N-acetylcysteine against fipronil-induced injuries: The antioxidant status and apoptotic markers expression in rats.
Artocarpin induces cell apoptosis in human osteosarcoma cells through endoplasmic reticulum stress and reactive oxygen species.
Benzyl isothiocyanate (BITC) triggers mitochondria-mediated apoptotic machinery in human cisplatin-resistant oral cancer CAR cells.
Melatonin Inhibits the Progression of Hepatocellular Carcinoma through MicroRNA Let7i-3p Mediated RAF1 Reduction.
Resveratrol inhibits human leiomyoma cell proliferation via crosstalk between integrin αvβ3 and IGF-1R.
Effects of carvacrol on human fibroblast (WS-1) and gastric adenocarcinoma (AGS) cells in vitro and on Wistar rats in vivo.
Pterostilbene modulates the suppression of multidrug resistance protein 1 and triggers autophagic and apoptotic mechanisms in cisplatin-resistant human oral cancer CAR cells via AKT signaling.
Metformin-induced caveolin-1 expression promotes T-DM1 drug efficacy in breast cancer cells.
Clinacanthus nutans Mitigates Neuronal Apoptosis and Ischemic Brain Damage Through Augmenting the C/EBPβ-Driven PPAR-γ Transcription.
FKBP12.6 protects heart from AngII-induced hypertrophy through inhibiting Ca(2+) /calmodulin-mediated signalling pathways in vivo and in vitro.
ITR‑284 modulates cell differentiation in human chronic myelogenous leukemia K562 cells.
Glycyrrhizic acid induces human MDA-MB-231 breast cancer cell death and autophagy via the ROS-mitochondrial pathway.
Neuroprotective Effects of Filgrastim in Rotenone-Induced Parkinson's Disease in Rats: Insights into its Anti-Inflammatory, Neurotrophic, and Antiapoptotic Effects.
Response of Myeloid Leukemia Cells to Luteolin is Modulated by Differentially Expressed Pituitary Tumor-Transforming Gene 1 (PTTG1) Oncoprotein.
The microRNA-302b-inhibited insulin-like growth factor-binding protein 2 signaling pathway induces glioma cell apoptosis by targeting nuclear factor IA.
Modulation of the mRNA-binding protein HuR as a novel reversal mechanism of epirubicin-triggered multidrug resistance in colorectal cancer cells.
Reactive oxygen species-driven mitochondrial injury induces apoptosis by teroxirone in human non-small cell lung cancer cells.
Isoliquiritigenin Induces Autophagy and Inhibits Ovarian Cancer Cell Growth.
Neferine Enhances the Antitumor Effect of Mitomycin-C in Hela Cells Through the Activation of p38-MAPK Pathway.
MicroRNA-target cross-talks: Key players in glioblastoma multiforme.
Therapeutic effect of urine-derived stem cells for protamine/lipopolysaccharide-induced interstitial cystitis in a rat model.
Rhodiola crenulata extract counteracts the effect of hypobaric hypoxia in rat heart via redirection of the nitric oxide and arginase 1 pathway.
DHEA protects mitochondria against dual modes of apoptosis and necroptosis in human granulosa HO23 cells.
High-Dose Fluoride Impairs the Properties of Human Embryonic Stem Cells via JNK Signaling.
The miR-204-3p-targeted IGFBP2 pathway is involved in xanthohumol-induced glioma cell apoptotic death.
Effects of Oxymatrine on the Proliferation and Apoptosis of Human Hepatoma Carcinoma Cells.
Urinary miR-16 transactivated by C/EBPβ reduces kidney function after ischemia/reperfusion-induced injury.
Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent and mitochondrial pathways in mammalian hepatic stellate cells.
Evaluation of the medicinal herb Graptopetalum paraguayense as a treatment for liver cancer.
PGRMC1 contributes to doxorubicin-induced chemoresistance in MES-SA uterine sarcoma.
MK-2206 induces apoptosis of AML cells and enhances the cytotoxicity of cytarabine.
Synergistic protection of N-acetylcysteine and ascorbic acid 2-phosphate on human mesenchymal stem cells against mitoptosis, necroptosis and apoptosis.
Paxillin promotes colorectal tumor invasion and poor patient outcomes via ERK-mediated stabilization of Bcl-2 protein by phosphorylation at Serine 87.
Generation of homologous cell pairs using the oral lymphatic system.
Acrylamide-induced apoptosis in rat primary astrocytes and human astrocytoma cell lines.
Omega-3 fatty acids, EPA and DHA induce apoptosis and enhance drug sensitivity in multiple myeloma cells but not in normal peripheral mononuclear cells
Phosphorylation of paxillin confers cisplatin resistance in non-small cell lung cancer via activating ERK-mediated Bcl-2 expression.
Cardioprotective Effects of Quercetin in Cardiomyocyte under Ischemia/Reperfusion Injury.
Stimulation of Toll-like receptor-1/2 combined with Velcade increases cytotoxicity to human multiple myeloma cells.
Momordica charantia Extract Induces Apoptosis in Human Cancer Cells through Caspase- and Mitochondria-Dependent Pathways.
Subamolide A, a component isolated from Cinnamomum subavenium, induces apoptosis mediated by mitochondria-dependent, p53 and ERK1/2 pathways in human urothelial carcinoma cell line NTUB1.
Ran S, Chu M, Gu S, Wang J, Liang J
International endodontic journal 2019 Jan;52(1):44-53
International endodontic journal 2019 Jan;52(1):44-53
Post-Injury Neuroprotective Effects of the Thalidomide Analog 3,6'-Dithiothalidomide on Traumatic Brain Injury.
Batsaikhan B, Wang JY, Scerba MT, Tweedie D, Greig NH, Miller JP, Hoffer BJ, Lin CT, Wang JY
International journal of molecular sciences 2019 Jan 24;20(3)
International journal of molecular sciences 2019 Jan 24;20(3)
Effects of ionizing radiation and HLY78 on the zebrafish embryonic developmental toxicity.
Si J, Zhou R, Zhao B, Xie Y, Gan L, Zhang J, Wang Y, Zhou X, Ren X, Zhang H
Toxicology 2019 Jan 1;411:143-153
Toxicology 2019 Jan 1;411:143-153
Effect of mild hypothermia on cerebral microcirculation in a murine cardiopulmonary resuscitation model.
Yu H, Wang L, Zhang H, Wei W, Chen Y, Tang W, Wan Z
Microcirculation (New York, N.Y. : 1994) 2019 Feb 23;
Microcirculation (New York, N.Y. : 1994) 2019 Feb 23;
Hepatorenal protective effects of taurine and N-acetylcysteine against fipronil-induced injuries: The antioxidant status and apoptotic markers expression in rats.
Abdel-Daim MM, Dessouki AA, Abdel-Rahman HG, Eltaysh R, Alkahtani S
The Science of the total environment 2019 Feb 10;650(Pt 2):2063-2073
The Science of the total environment 2019 Feb 10;650(Pt 2):2063-2073
Artocarpin induces cell apoptosis in human osteosarcoma cells through endoplasmic reticulum stress and reactive oxygen species.
Lee CW, Chi MC, Chang TM, Liu JF
Journal of cellular physiology 2019 Aug;234(8):13157-13168
Journal of cellular physiology 2019 Aug;234(8):13157-13168
Benzyl isothiocyanate (BITC) triggers mitochondria-mediated apoptotic machinery in human cisplatin-resistant oral cancer CAR cells.
Lee CF, Chiang NN, Lu YH, Huang YS, Yang JS, Tsai SC, Lu CC, Chen FA
BioMedicine 2018 Sep;8(3):15
BioMedicine 2018 Sep;8(3):15
Melatonin Inhibits the Progression of Hepatocellular Carcinoma through MicroRNA Let7i-3p Mediated RAF1 Reduction.
Wang TH, Hsueh C, Chen CC, Li WS, Yeh CT, Lian JH, Chang JL, Chen CY
International journal of molecular sciences 2018 Sep 10;19(9)
International journal of molecular sciences 2018 Sep 10;19(9)
Resveratrol inhibits human leiomyoma cell proliferation via crosstalk between integrin αvβ3 and IGF-1R.
Ho Y, Sh Yang YC, Chin YT, Chou SY, Chen YR, Shih YJ, Whang-Peng J, Changou CA, Liu HL, Lin SJ, Tang HY, Lin HY, Davis PJ
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2018 Oct;120:346-355
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2018 Oct;120:346-355
Effects of carvacrol on human fibroblast (WS-1) and gastric adenocarcinoma (AGS) cells in vitro and on Wistar rats in vivo.
Günes-Bayir A, Kocyigit A, Güler EM, Bilgin MG, Ergün İS, Dadak A
Molecular and cellular biochemistry 2018 Nov;448(1-2):237-249
Molecular and cellular biochemistry 2018 Nov;448(1-2):237-249
Pterostilbene modulates the suppression of multidrug resistance protein 1 and triggers autophagic and apoptotic mechanisms in cisplatin-resistant human oral cancer CAR cells via AKT signaling.
Chang HP, Lu CC, Chiang JH, Tsai FJ, Juan YN, Tsao JW, Chiu HY, Yang JS
International journal of oncology 2018 May;52(5):1504-1514
International journal of oncology 2018 May;52(5):1504-1514
Metformin-induced caveolin-1 expression promotes T-DM1 drug efficacy in breast cancer cells.
Chung YC, Chang CM, Wei WC, Chang TW, Chang KJ, Chao WT
Scientific reports 2018 Mar 2;8(1):3930
Scientific reports 2018 Mar 2;8(1):3930
Clinacanthus nutans Mitigates Neuronal Apoptosis and Ischemic Brain Damage Through Augmenting the C/EBPβ-Driven PPAR-γ Transcription.
Wu JS, Kao MH, Tsai HD, Cheung WM, Chen JJ, Ong WY, Sun GY, Lin TN
Molecular neurobiology 2018 Jul;55(7):5425-5438
Molecular neurobiology 2018 Jul;55(7):5425-5438
FKBP12.6 protects heart from AngII-induced hypertrophy through inhibiting Ca(2+) /calmodulin-mediated signalling pathways in vivo and in vitro.
Xiao YF, Zeng ZX, Guan XH, Wang LF, Wang CJ, Shi H, Shou W, Deng KY, Xin HB
Journal of cellular and molecular medicine 2018 Jul;22(7):3638-3651
Journal of cellular and molecular medicine 2018 Jul;22(7):3638-3651
ITR‑284 modulates cell differentiation in human chronic myelogenous leukemia K562 cells.
Yang JS, Lee CY, Cho HC, Lu CC, Kuo SC, Wen YF, Tsai FJ, Lee MR, Tsai SC
Oncology reports 2018 Jan;39(1):383-391
Oncology reports 2018 Jan;39(1):383-391
Glycyrrhizic acid induces human MDA-MB-231 breast cancer cell death and autophagy via the ROS-mitochondrial pathway.
Lin SC, Chu PY, Liao WT, Wu MY, Tsui KH, Lin LT, Huang CH, Chen LL, Li CJ
Oncology reports 2018 Feb;39(2):703-710
Oncology reports 2018 Feb;39(2):703-710
Neuroprotective Effects of Filgrastim in Rotenone-Induced Parkinson's Disease in Rats: Insights into its Anti-Inflammatory, Neurotrophic, and Antiapoptotic Effects.
Azmy MS, Menze ET, El-Naga RN, Tadros MG
Molecular neurobiology 2018 Aug;55(8):6572-6588
Molecular neurobiology 2018 Aug;55(8):6572-6588
Response of Myeloid Leukemia Cells to Luteolin is Modulated by Differentially Expressed Pituitary Tumor-Transforming Gene 1 (PTTG1) Oncoprotein.
Chen PY, Tien HJ, Chen SF, Horng CT, Tang HL, Jung HL, Wu MJ, Yen JH
International journal of molecular sciences 2018 Apr 12;19(4)
International journal of molecular sciences 2018 Apr 12;19(4)
The microRNA-302b-inhibited insulin-like growth factor-binding protein 2 signaling pathway induces glioma cell apoptosis by targeting nuclear factor IA.
Lee CC, Chen PH, Ho KH, Shih CM, Cheng CH, Lin CW, Cheng KT, Liu AJ, Chen KC
PloS one 2017;12(3):e0173890
PloS one 2017;12(3):e0173890
Modulation of the mRNA-binding protein HuR as a novel reversal mechanism of epirubicin-triggered multidrug resistance in colorectal cancer cells.
Lin GL, Ting HJ, Tseng TC, Juang V, Lo YL
PloS one 2017;12(10):e0185625
PloS one 2017;12(10):e0185625
Reactive oxygen species-driven mitochondrial injury induces apoptosis by teroxirone in human non-small cell lung cancer cells.
Wang JP, Hsieh CH, Liu CY, Lin KH, Wu PT, Chen KM, Fang K
Oncology letters 2017 Sep;14(3):3503-3509
Oncology letters 2017 Sep;14(3):3503-3509
Isoliquiritigenin Induces Autophagy and Inhibits Ovarian Cancer Cell Growth.
Chen HY, Huang TC, Shieh TM, Wu CH, Lin LC, Hsia SM
International journal of molecular sciences 2017 Sep 21;18(10)
International journal of molecular sciences 2017 Sep 21;18(10)
Neferine Enhances the Antitumor Effect of Mitomycin-C in Hela Cells Through the Activation of p38-MAPK Pathway.
Eid W, Abdel-Rehim W
Journal of cellular biochemistry 2017 Oct;118(10):3472-3479
Journal of cellular biochemistry 2017 Oct;118(10):3472-3479
MicroRNA-target cross-talks: Key players in glioblastoma multiforme.
Toraih EA, Aly NM, Abdallah HY, Al-Qahtani SA, Shaalan AA, Hussein MH, Fawzy MS
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2017 Nov;39(11):1010428317726842
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2017 Nov;39(11):1010428317726842
Therapeutic effect of urine-derived stem cells for protamine/lipopolysaccharide-induced interstitial cystitis in a rat model.
Li J, Luo H, Dong X, Liu Q, Wu C, Zhang T, Hu X, Zhang Y, Song B, Li L
Stem cell research & therapy 2017 May 8;8(1):107
Stem cell research & therapy 2017 May 8;8(1):107
Rhodiola crenulata extract counteracts the effect of hypobaric hypoxia in rat heart via redirection of the nitric oxide and arginase 1 pathway.
Hsu SW, Chang TC, Wu YK, Lin KT, Shi LS, Lee SY
BMC complementary and alternative medicine 2017 Jan 7;17(1):29
BMC complementary and alternative medicine 2017 Jan 7;17(1):29
DHEA protects mitochondria against dual modes of apoptosis and necroptosis in human granulosa HO23 cells.
Tsui KH, Wang PH, Lin LT, Li CJ
Reproduction (Cambridge, England) 2017 Aug;154(2):101-110
Reproduction (Cambridge, England) 2017 Aug;154(2):101-110
High-Dose Fluoride Impairs the Properties of Human Embryonic Stem Cells via JNK Signaling.
Fu X, Xie FN, Dong P, Li QC, Yu GY, Xiao R
PloS one 2016;11(2):e0148819
PloS one 2016;11(2):e0148819
The miR-204-3p-targeted IGFBP2 pathway is involved in xanthohumol-induced glioma cell apoptotic death.
Chen PH, Chang CK, Shih CM, Cheng CH, Lin CW, Lee CC, Liu AJ, Ho KH, Chen KC
Neuropharmacology 2016 Nov;110(Pt A):362-375
Neuropharmacology 2016 Nov;110(Pt A):362-375
Effects of Oxymatrine on the Proliferation and Apoptosis of Human Hepatoma Carcinoma Cells.
Liu Y, Bi T, Dai W, Wang G, Qian L, Gao Q, Shen G
Technology in cancer research & treatment 2016 Jun;15(3):487-97
Technology in cancer research & treatment 2016 Jun;15(3):487-97
Urinary miR-16 transactivated by C/EBPβ reduces kidney function after ischemia/reperfusion-induced injury.
Chen HH, Lan YF, Li HF, Cheng CF, Lai PF, Li WH, Lin H
Scientific reports 2016 Jun 14;6:27945
Scientific reports 2016 Jun 14;6:27945
Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent and mitochondrial pathways in mammalian hepatic stellate cells.
Chen MF, Huang SJ, Huang CC, Liu PS, Lin KI, Liu CW, Hsieh WC, Shiu LY, Chen CH
BMC cancer 2016 Jul 26;16:532
BMC cancer 2016 Jul 26;16:532
Evaluation of the medicinal herb Graptopetalum paraguayense as a treatment for liver cancer.
Hsu WH, Chang CC, Huang KW, Chen YC, Hsu SL, Wu LC, Tsou AP, Lai JM, Huang CY
PloS one 2015;10(4):e0121298
PloS one 2015;10(4):e0121298
PGRMC1 contributes to doxorubicin-induced chemoresistance in MES-SA uterine sarcoma.
Lin ST, May EW, Chang JF, Hu RY, Wang LH, Chan HL
Cellular and molecular life sciences : CMLS 2015 Jun;72(12):2395-409
Cellular and molecular life sciences : CMLS 2015 Jun;72(12):2395-409
MK-2206 induces apoptosis of AML cells and enhances the cytotoxicity of cytarabine.
Lu JW, Lin YM, Lai YL, Chen CY, Hu CY, Tien HF, Ou DL, Lin LI
Medical oncology (Northwood, London, England) 2015 Jul;32(7):206
Medical oncology (Northwood, London, England) 2015 Jul;32(7):206
Synergistic protection of N-acetylcysteine and ascorbic acid 2-phosphate on human mesenchymal stem cells against mitoptosis, necroptosis and apoptosis.
Li CJ, Sun LY, Pang CY
Scientific reports 2015 Apr 24;5:9819
Scientific reports 2015 Apr 24;5:9819
Paxillin promotes colorectal tumor invasion and poor patient outcomes via ERK-mediated stabilization of Bcl-2 protein by phosphorylation at Serine 87.
Huang CC, Wu DW, Lin PL, Lee H
Oncotarget 2015 Apr 20;6(11):8698-708
Oncotarget 2015 Apr 20;6(11):8698-708
Generation of homologous cell pairs using the oral lymphatic system.
Pu YF, Wang L, Wu HH, Bian H, Hong YY, Wang YX, Guo CB
International journal of clinical and experimental pathology 2014;7(4):1563-71
International journal of clinical and experimental pathology 2014;7(4):1563-71
Acrylamide-induced apoptosis in rat primary astrocytes and human astrocytoma cell lines.
Lee JG, Wang YS, Chou CC
Toxicology in vitro : an international journal published in association with BIBRA 2014 Jun;28(4):562-70
Toxicology in vitro : an international journal published in association with BIBRA 2014 Jun;28(4):562-70
Omega-3 fatty acids, EPA and DHA induce apoptosis and enhance drug sensitivity in multiple myeloma cells but not in normal peripheral mononuclear cells
Abdi J, Garssen J, Faber J, Redegeld F
The Journal of Nutritional Biochemistry 2014 December;25(12):1254-1262
The Journal of Nutritional Biochemistry 2014 December;25(12):1254-1262
Phosphorylation of paxillin confers cisplatin resistance in non-small cell lung cancer via activating ERK-mediated Bcl-2 expression.
Wu DW, Wu TC, Wu JY, Cheng YW, Chen YC, Lee MC, Chen CY, Lee H
Oncogene 2014 Aug 28;33(35):4385-95
Oncogene 2014 Aug 28;33(35):4385-95
Cardioprotective Effects of Quercetin in Cardiomyocyte under Ischemia/Reperfusion Injury.
Chen YW, Chou HC, Lin ST, Chen YH, Chang YJ, Chen L, Chan HL
Evidence-based complementary and alternative medicine : eCAM 2013;2013:364519
Evidence-based complementary and alternative medicine : eCAM 2013;2013:364519
Stimulation of Toll-like receptor-1/2 combined with Velcade increases cytotoxicity to human multiple myeloma cells.
Abdi J, Mutis T, Garssen J, Redegeld F
Blood cancer journal 2013 May 31;3:e119
Blood cancer journal 2013 May 31;3:e119
Momordica charantia Extract Induces Apoptosis in Human Cancer Cells through Caspase- and Mitochondria-Dependent Pathways.
Li CJ, Tsang SF, Tsai CH, Tsai HY, Chyuan JH, Hsu HY
Evidence-based complementary and alternative medicine : eCAM 2012;2012:261971
Evidence-based complementary and alternative medicine : eCAM 2012;2012:261971
Subamolide A, a component isolated from Cinnamomum subavenium, induces apoptosis mediated by mitochondria-dependent, p53 and ERK1/2 pathways in human urothelial carcinoma cell line NTUB1.
Liu CH, Chen CY, Huang AM, Li JH
Journal of ethnopharmacology 2011 Sep 1;137(1):503-11
Journal of ethnopharmacology 2011 Sep 1;137(1):503-11
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Bcl-2 antibody detects Bcl-2 protein by western blot analysis. A. 30 ?g 293T whole cell lysate/extract B. 30 ?g whole cell lysate/extract of human Bcl-2-transfected 293T cells 12% SDS PAGE Bcl-2 antibody (GTX100064) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using Bcl-2(GTX100064) antibody at 1:250 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Bcl-2 antibody [N1N2], N-term detects Bcl-2 protein at cytoplasm in human squamous metaplasia of cervix by immunohistochemical analysis. Sample: Paraffin-embedded human cervix. Bcl-2 antibody [N1N2], N-term (GTX100064) diluted at 1:500.
