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- Antibody Data
- Antigen structure
- References [21]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- V2007 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Bcl-2 Antibody
- Antibody type
- Monoclonal
- Antigen
- Amino acids GAAPAPGIFSSQPG (41-54) of the human protein were used as the immunogen for this Bcl-2 antibody.
- Description
- Protein G purified antibody
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 100/D5
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- Store the Bcl-2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Epigallocatechin-3-gallate prevents cardiac apoptosis by modulating the intrinsic apoptotic pathway in isoproterenol-induced myocardial infarction.
The role of immunohistochemical adrenomedullin and Bcl-2 expression in development of type-1 endometrial adenocarcinoma: Adrenomedullin expression in endometrium.
Immunohistochemical overexpression of hypoxia-induced factor 1α associated with slow reduction in 18fluoro-2-deoxy-D-glucose uptake for chemoradiotherapy in patients with pharyngeal cancer.
The Role of Fast Cell Cycle Analysis in Pediatric Brain Tumors.
Pattern of MEF2B expression in lymphoid tissues and in malignant lymphomas.
FOXA2 mRNA expression is associated with relapse in patients with Triple-Negative/Basal-like breast carcinoma.
Hypoxia-inducible factor-1α, adrenomedullin and Bcl-2 although expected are not related to increased uptake of fluorine-18-fluorodeoxyglucose in endometrial cancer.
Clinical and pathological features of Burkitt lymphoma showing expression of BCL2--an analysis including gene expression in formalin-fixed paraffin-embedded tissue.
Metachronous cardiac and cerebral sarcomas: case report with focus on molecular findings and review of the literature.
Gastric cancer in young vs old Romanian patients: immunoprofile with emphasis on maspin and mena protein reactivity.
Polyphenon-60 ameliorates metabolic risk factors, oxidative stress, and proinflammatory cytokines and modulates apoptotic proteins to protect the heart against streptozotocin-induced apoptosis
A rare case of monophasic synovial sarcoma of the hand: Cytological and immunohistopathological study
Lack of relation of AKAP12 with p53 and Bcl-2 in colorectal carcinoma.
Aberrant immunoarchitecture distinguishes hyperplastic germinal centres in pattern 1 angioimmunoblastic T-cell lymphoma from reactive follicles.
Association of increased osteopontin and splice variant-c mRNA expression with HER2 and triple-negative/basal-like breast carcinomas subtypes and recurrence.
Primary bone marrow diffuse large B-cell lymphoma accompanying cold agglutinin disease: A case report with review of the literature.
Prognostic significance of p16, p53, Bcl-2, and Bax in oral and oropharyngeal squamous cell carcinoma
Asian Biomedicine 2014 April
Sphingosine kinase activity counteracts ceramide-mediated cell death in human melanoma cells: role of Bcl-2 expression.
Expression of biomolecular markers (Ki-67, PCNA, Bcl-2, BAX, BclX, VEGF) in breast tumors.
Cyclooxygenase-2 expression correlates with angiogenesis and apoptosis in gastric cancer tissue.
The Bax/Bcl-2 ratio determines the susceptibility of human melanoma cells to CD95/Fas-mediated apoptosis.
Othman AI, Elkomy MM, El-Missiry MA, Dardor M
European journal of pharmacology 2017 Jan 5;794:27-36
European journal of pharmacology 2017 Jan 5;794:27-36
The role of immunohistochemical adrenomedullin and Bcl-2 expression in development of type-1 endometrial adenocarcinoma: Adrenomedullin expression in endometrium.
Bozkurt KK, Yalçın Y, Erdemoğlu E, Tatar B, Erdemoğlu E, Çerçi SS, Çiriş İM, Başpınar Ş, Uğuz A, Kapucuoğlu N
Pathology, research and practice 2016 May;212(5):450-5
Pathology, research and practice 2016 May;212(5):450-5
Immunohistochemical overexpression of hypoxia-induced factor 1α associated with slow reduction in 18fluoro-2-deoxy-D-glucose uptake for chemoradiotherapy in patients with pharyngeal cancer.
Chen SW, Lin YC, Chen RY, Hsieh TC, Yen KY, Liang JA, Yang SN, Wang YC, Chen YH, Chow NH, Kao CH
European journal of nuclear medicine and molecular imaging 2016 Dec;43(13):2343-2352
European journal of nuclear medicine and molecular imaging 2016 Dec;43(13):2343-2352
The Role of Fast Cell Cycle Analysis in Pediatric Brain Tumors.
Alexiou GA, Vartholomatos G, Stefanaki K, Lykoudis EG, Patereli A, Tseka G, Tzoufi M, Sfakianos G, Prodromou N
Pediatric neurosurgery 2015;50(5):257-63
Pediatric neurosurgery 2015;50(5):257-63
Pattern of MEF2B expression in lymphoid tissues and in malignant lymphomas.
Krenács D, Borbényi Z, Bedekovics J, Méhes G, Bagdi E, Krenács L
Virchows Archiv : an international journal of pathology 2015 Sep;467(3):345-55
Virchows Archiv : an international journal of pathology 2015 Sep;467(3):345-55
FOXA2 mRNA expression is associated with relapse in patients with Triple-Negative/Basal-like breast carcinoma.
Perez-Balaguer A, Ortiz-Martínez F, García-Martínez A, Pomares-Navarro C, Lerma E, Peiró G
Breast cancer research and treatment 2015 Sep;153(2):465-74
Breast cancer research and treatment 2015 Sep;153(2):465-74
Hypoxia-inducible factor-1α, adrenomedullin and Bcl-2 although expected are not related to increased uptake of fluorine-18-fluorodeoxyglucose in endometrial cancer.
Cerci SS, Yalcin Y, Bozkurt KK, Erdemoglu E, Tatar B, Erdemoglu E
Hellenic journal of nuclear medicine 2015 Sep-Dec;18(3):228-32
Hellenic journal of nuclear medicine 2015 Sep-Dec;18(3):228-32
Clinical and pathological features of Burkitt lymphoma showing expression of BCL2--an analysis including gene expression in formalin-fixed paraffin-embedded tissue.
Masqué-Soler N, Szczepanowski M, Kohler CW, Aukema SM, Nagel I, Richter J, Siebert R, Spang R, Burkhardt B, Klapper W
British journal of haematology 2015 Nov;171(4):501-8
British journal of haematology 2015 Nov;171(4):501-8
Metachronous cardiac and cerebral sarcomas: case report with focus on molecular findings and review of the literature.
Guerriero A, Giovenali P, La Starza R, Mecucci C, Montesi G, Pasquino S, Pierini T, Ragni T, Sidoni A
Human pathology 2015 Mar;46(3):482-7
Human pathology 2015 Mar;46(3):482-7
Gastric cancer in young vs old Romanian patients: immunoprofile with emphasis on maspin and mena protein reactivity.
Gurzu S, Kadar Z, Sugimura H, Bara T, Bara T Jr, Halmaciu I, Jung I
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2015 Mar;123(3):223-33
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2015 Mar;123(3):223-33
Polyphenon-60 ameliorates metabolic risk factors, oxidative stress, and proinflammatory cytokines and modulates apoptotic proteins to protect the heart against streptozotocin-induced apoptosis
El-Missiry M, Amer M, Othman A, Yaseen khashman
Egyptian Journal of Basic and Applied Sciences 2015 June;2(2):120-131
Egyptian Journal of Basic and Applied Sciences 2015 June;2(2):120-131
A rare case of monophasic synovial sarcoma of the hand: Cytological and immunohistopathological study
Siddhi Gaurish S, Zode A, Avinash J, Kulkarni M, Patel D
Oncology, Gastroenterology and Hepatology Reports 2015 ;4(2):116
Oncology, Gastroenterology and Hepatology Reports 2015 ;4(2):116
Lack of relation of AKAP12 with p53 and Bcl-2 in colorectal carcinoma.
Suren D, Yildirim M, Alikanoglu AS, Kaya V, Yildiz M, Dilli UD, Sezer C
Asian Pacific journal of cancer prevention : APJCP 2014;15(8):3415-8
Asian Pacific journal of cancer prevention : APJCP 2014;15(8):3415-8
Aberrant immunoarchitecture distinguishes hyperplastic germinal centres in pattern 1 angioimmunoblastic T-cell lymphoma from reactive follicles.
Tan LH, Tan SY
Hematological oncology 2014 Sep;32(3):145-54
Hematological oncology 2014 Sep;32(3):145-54
Association of increased osteopontin and splice variant-c mRNA expression with HER2 and triple-negative/basal-like breast carcinomas subtypes and recurrence.
Ortiz-Martínez F, Perez-Balaguer A, Ciprián D, Andrés L, Ponce J, Adrover E, Sánchez-Payá J, Aranda FI, Lerma E, Peiró G
Human pathology 2014 Mar;45(3):504-12
Human pathology 2014 Mar;45(3):504-12
Primary bone marrow diffuse large B-cell lymphoma accompanying cold agglutinin disease: A case report with review of the literature.
Yamashita T, Ishida M, Moro H, Yumoto H, Uchibayashi S, Yoshii M, Nakanishi R, Okuno H, Yoshida T, Okuno T, Hodohara K, Okabe H
Oncology letters 2014 Jan;7(1):79-81
Oncology letters 2014 Jan;7(1):79-81
Prognostic significance of p16, p53, Bcl-2, and Bax in oral and oropharyngeal squamous cell carcinoma
Asian Biomedicine 2014 April
Sphingosine kinase activity counteracts ceramide-mediated cell death in human melanoma cells: role of Bcl-2 expression.
Bektas M, Jolly PS, Müller C, Eberle J, Spiegel S, Geilen CC
Oncogene 2005 Jan 6;24(1):178-87
Oncogene 2005 Jan 6;24(1):178-87
Expression of biomolecular markers (Ki-67, PCNA, Bcl-2, BAX, BclX, VEGF) in breast tumors.
Kushlinskii NE, Orinovskii MB, Gurevich LE, Kazantseva IA, Talaeva ShZh, Shirokii VP, Ermilova VD, Dvorova EK, Ozherelev AS
Bulletin of experimental biology and medicine 2004 Feb;137(2):182-5
Bulletin of experimental biology and medicine 2004 Feb;137(2):182-5
Cyclooxygenase-2 expression correlates with angiogenesis and apoptosis in gastric cancer tissue.
Tatsuguchi A, Matsui K, Shinji Y, Gudis K, Tsukui T, Kishida T, Fukuda Y, Sugisaki Y, Tokunaga A, Tajiri T, Sakamoto C
Human pathology 2004 Apr;35(4):488-95
Human pathology 2004 Apr;35(4):488-95
The Bax/Bcl-2 ratio determines the susceptibility of human melanoma cells to CD95/Fas-mediated apoptosis.
Raisova M, Hossini AM, Eberle J, Riebeling C, Wieder T, Sturm I, Daniel PT, Orfanos CE, Geilen CC
The Journal of investigative dermatology 2001 Aug;117(2):333-40
The Journal of investigative dermatology 2001 Aug;117(2):333-40
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human samples using 100/D5 antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of formalin-paraffin non-Hodgkin's lymphoma stained with Bcl-2 antibody (100/D5). Note nuclear membrane & cytoplasmic staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS staining (intracellular) of Jurkat cells using 100/D5 antibody (red) and isotype control (green).
