V2010
antibody from NSJ Bioreagents
Targeting: BCL2
Bcl-2, PPP1R50
Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- V2010 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Bcl2 Antibody Cocktail
- Antibody type
- Monoclonal
- Antigen
- A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG) of the human protein was used as the immunogen for both antibodies in this Bcl2 antibody cocktail.
- Description
- Protein G purified antibody
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 100/D5 + 124
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- Store the Bcl2 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Primary follicular lymphoma of the epididymis positive for t(14;18)(q32;q21)/IGH-BCL2 and negative for BCL2 protein expression: a case report.
Comparative clinicopathologic and immunohistochemical analysis of uterine sarcomas diagnosed using the World Health Organization classification system.
Tralongo V, Becchina G, Nagar C, Ottoveggio G, Mason S, Seno B, Sessa F, Frattini M, Genovese F
Journal of medical case reports 2012 Jan 18;6:24
Journal of medical case reports 2012 Jan 18;6:24
Comparative clinicopathologic and immunohistochemical analysis of uterine sarcomas diagnosed using the World Health Organization classification system.
D'Angelo E, Spagnoli LG, Prat J
Human pathology 2009 Nov;40(11):1571-85
Human pathology 2009 Nov;40(11):1571-85
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human samples using Bcl2 antibody cocktail.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE non-Hodgkin's lymphoma stained with Bcl2 antibody cocktail.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS staining (intracellular) of Jurkat cells using Bcl2 antibody cocktail (red) and isotype control (green).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free Bcl2 Antibody cocktail (100/D5 + 124). Confirmation of Integrity and Purity of the Antibody.