Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 200-401-222 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-401-222, RRID:AB_2064309
- Product name
- Anti-BCL2 (RABBIT) Antibody - 200-401-222
- Antibody type
- Polyclonal
- Vial size
- 200 µl
Submitted references Peptidomimetic blockade of MYB in acute myeloid leukemia.
Ramaswamy K, Forbes L, Minuesa G, Gindin T, Brown F, Kharas MG, Krivtsov AV, Armstrong SA, Still E, de Stanchina E, Knoechel B, Koche R, Kentsis A
Nature communications 2018 Jan 9;9(1):110
Nature communications 2018 Jan 9;9(1):110
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Anti-Bcl-2 is shown to detect Bcl-2 in GP-E86 cell whole cell lysates. Lanes 1 -10 and 12 are bcl-2 mutants. Lane 11 is wt bcl-2. Lane 13 is an empty vector lysate. Detection occurs using a 1:1,000 dilution of antibody followed by 1:4,000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1. Other detection systems will yield similar results.
- Validation comment
- Western Blot