60178-1-IG

antibody from Invitrogen Antibodies

Targeting: BCL2 Bcl-2, PPP1R50

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

60178-1-IG

Provider

Invitrogen Antibodies

Product name

BCL2 Monoclonal Antibody (4H8C6)

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: MAHAGRTGY DNREIVMKYI HYKLSQRGYE WDAGDVGAAP PGAAPAPGIF SSQPGHTPHP AASRDPVART SPLQTPAAPG AAAGPALSPV PPVVHLTLRQ AGDDFSRRYR RDFAEMSSQL HLTPFTARGR FATVVEELFR DGVNWGRIVA FFEFGGVMCV ESVNREMSPL VDNIALWMTE YLNRHLHTWI QDNGGWDAFV ELYGPSMRPL FDFSWLSLKT LLSLALVGAC ITLGAYLGHK (1-239 aa encoded by BC027258)

Reactivity

Human, Porcine

Host

Mouse

Isotype

IgG

Antibody clone number

4H8C6

Vial size

150 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 60178-1-IG (BCL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of BCL2 antibody (60178-1-IG, 1:1000) with si-control and si-BCL2 transfected Jurkat cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 60178-1-Ig (BCL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 60178-1-Ig (BCL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Pig spleen tissue were subjected to SDS PAGE followed by western blot with 60178-1-Ig (BCL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

U-937 cells were subjected to SDS PAGE followed by western blot with 60178-1-Ig (BCL2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol) fixed MCF-7 cells using 60178-1-IG (BCL2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human APpendicitis tissue slide using 60178-1-IG (BCL2 antibody) at dilution of 1:12800 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human APpendicitis tissue slide using 60178-1-IG (BCL2 antibody) at dilution of 1:12800 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:12800 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 60178-1-Ig (BCL2 Antibody) at dilution of 1:3000 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:12800 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:12800 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:12800 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 60178-1-Ig (BCL2 Antibody) at dilution of 1:1000 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 60178-1-Ig (BCL2 Antibody) at dilution of 1:1000 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 60178-1-Ig (BCL2 Antibody) at dilution of 1:3000 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 60178-1-Ig (BCL2 Antibody) at dilution of 1:3000 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 60178-1-Ig (BCL2 Antibody) at dilution of 1:3000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 human peripheral blood lymphocytes were stained with 0.2 µg Anti-Human BCL2 (Product # 60178-1-IG, Clone:4H8C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1,000 (green), or 0.2 µg isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1,000 (black). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 Jurkat cells were stained with 0.2ug BCL2 antibody (60178-1-IG, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L) with dilution 1:1000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-BCL2 (IP: 60178-1-IG, 5 µg; Detection: 60178-1-IG 1:1000) with U-937 cells lysate 4000 µg.