Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-114929 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CGRP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CGRP in mouse lung (left lane: treated with antigen-specific peptide). Samples were incubated with polyclonal antibody (Product # PA5-114929).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CGRP in MCF7 cells (left lane: treated with blocking peptide). Samples were incubated with polyclonal antibody (Product # PA5-114929).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of CGRP in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114929) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CGRP in human uterus tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114929) at a dilution of 1:100. Secondary staining was applied with HRP conjugated goat anti-rabbit.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CGRP in human colorectal cancer and adjacent normal tissues. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-114929) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.