Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP32742PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti PPP3R1 / Calcineurin B
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide (KASYPLEMCSHFD) coupled to KLH.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 0.1 ml
- Concentration
- 0.7 mg/ml (Lot specific)
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blot Analysis: A431 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Calcineurin B Antibody (1/500 dilution of this lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein Calcineurin Bα (~19 kDa).Â
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry on Paraffin Sections: Calcineurin Bα representative staining pattern/morphology in normal human placenta. Tissue was pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1/100, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seen here in nutrient rich erythrocytes for both mother and embryo blood.