H00005534-M01

antibody from Abnova Corporation

Targeting: PPP3R1 CALNB1, CNB, CNB1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005534-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005534-M01, RRID:AB_425610

Product name

PPP3R1 monoclonal antibody (M01), clone 4E1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant PPP3R1.

Antigen sequence

MGNEASYPLEMCSHFDADEIKRLGKRFKKLDLDNS
GSLSVEEFMSLPELQQNPLVQRVIDIFDTDGNGEV
DFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDG
YISNGELFQVLKMMVGNNLKDTQLQQIVDKTIINA
DKDGDGRISFEEFCAVVGGLDIHKKMVVDV


Isotype

IgG

Antibody clone number

4E1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Placenta-specific miRNA (miR-512-3p) targets PPP3R1 encoding the calcineurin B regulatory subunit in BeWo cells.

Kurashina R, Kikuchi K, Iwaki J, Yoshitake H, Takeshita T, Takizawa T
The journal of obstetrics and gynaecology research 2014 Mar;40(3):650-60

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

PPP3R1 monoclonal antibody (M01), clone 4E1. Western Blot analysis of PPP3R1 expression in rat brain.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PPP3R1 is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to PPP3R1 on HeLa cell. [antibody concentration 25 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol