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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00005534-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005534-M01, RRID:AB_547263
- Product name
- Mouse Monoclonal Calcineurin B Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PPP3R1 - protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I)
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Placenta-specific miRNA (miR-512-3p) targets PPP3R1 encoding the calcineurin B regulatory subunit in BeWo cells.
Kurashina R, Kikuchi K, Iwaki J, Yoshitake H, Takeshita T, Takizawa T
The journal of obstetrics and gynaecology research 2014 Mar;40(3):650-60
The journal of obstetrics and gynaecology research 2014 Mar;40(3):650-60
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Calcineurin B Antibody (4E1) [H00005534-M01] - PPP3R1 monoclonal antibody (M01), clone 4E1. Analysis of PPP3R1 expression in rat brain.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Calcineurin B Antibody (4E1.) [H00005534-M01] - Calcineurin B Antibody (4E1) [H00005534-M01] - Detection limit for recombinant GST tagged PPP3R1 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Calcineurin B Antibody (4E1) [H00005534-M01] - Analysis of monoclonal antibody to PPP3R1 on HeLa cell. Antibody concentration 25 ug/ml.
