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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-29939 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Calcineurin B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7, mouse fetal brain, rat brain, MEF.
- Concentration
- 0.76 mg/mL
Submitted references A rare genomic duplication in 2p14 underlies autosomal dominant hearing loss DFNA58.
Lezirovitz K, Vieira-Silva GA, Batissoco AC, Levy D, Kitajima JP, Trouillet A, Ouyang E, Zebarjadi N, Sampaio-Silva J, Pedroso-Campos V, Nascimento LR, Sonoda CY, Borges VM, Vasconcelos LG, Beck RMO, Grasel SS, Jagger DJ, Grillet N, Bento RF, Mingroni-Netto RC, Oiticica J
Human molecular genetics 2020 Jun 3;29(9):1520-1536
Human molecular genetics 2020 Jun 3;29(9):1520-1536
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extract (30 µg lysate) of IMR-32 (Lane 1), SH-SY5Y (Lane 2), MCF7 (Lane 3), MOLT-4 (Lane 4), HeLa (Lane 5), SW480 (Lane 6), tissue extracts of Mouse Brain (Lane 7) and Rat Brain (Lane 8). The blot was probed with Anti-Calcineurin B Polyclonal Antibody (Product # PA5-29939, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 19 kDa band corresponding to Calcineurin B was observed across the cell lines and tissues tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Calcineurin B was performed by separating 50 µg of rat tissue extract by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a Calcineurin B Polyclonal Antibody (Product # PA5-29939) at a dilution of 1:50,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Calcineurin B Polyclonal Antibody (Product # PA5-29939). Sample (30 µg of whole cell lysate). A: IMR32 . 12% SDS PAGE. Calcineurin B Polyclonal Antibody (Product # PA5-29939) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Calcineurin B was achieved by transfecting HeLa with Calcineurin B specific siRNAs (Silencer® select Product # s193, s192). Western blot analysis (Fig. a) was performed using whole cell extracts from the Calcineurin B knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Calcineurin B Polyclonal Antibody (Product # PA5-29939, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Reduction of signal upon siRNA mediated knock down confirms that antibody is specific to Calcineurin B.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Calcineurin B was performed in DIV9 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Calcineurin B Polyclonal Antibody (Product # PA5 29939) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Calcineurin B (Product # PA5-29939) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
