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- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [2]
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- Product number
- AF1348 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Calcineurin B Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human, mouse, and rat Calcineurin B in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
P63098- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Combinatory FK506 and Minocycline Treatment Alleviates Prion-Induced Neurodegenerative Events via Caspase-Mediated MAPK-NRF2 Pathway.
MicroRNA interactome analysis predicts post-transcriptional regulation of ADRB2 and PPP3R1 in the hypercholesterolemic myocardium.
Phosphatase inhibition increases AQP2 accumulation in the rat IMCD apical plasma membrane.
Regulation of RCAN1 protein activity by Dyrk1A protein-mediated phosphorylation.
Shah SZA, Zhao D, Taglialatela G, Hussain T, Dong H, Sabir N, Mangi MH, Wu W, Lai M, Zhang X, Duan Y, Wang L, Zhou X, Yang L
International journal of molecular sciences 2019 Mar 6;20(5)
International journal of molecular sciences 2019 Mar 6;20(5)
MicroRNA interactome analysis predicts post-transcriptional regulation of ADRB2 and PPP3R1 in the hypercholesterolemic myocardium.
Ágg B, Baranyai T, Makkos A, Vető B, Faragó N, Zvara Á, Giricz Z, Veres DV, Csermely P, Arányi T, Puskás LG, Varga ZV, Ferdinandy P
Scientific reports 2018 Jul 4;8(1):10134
Scientific reports 2018 Jul 4;8(1):10134
Phosphatase inhibition increases AQP2 accumulation in the rat IMCD apical plasma membrane.
Ren H, Yang B, Ruiz JA, Efe O, Ilori TO, Sands JM, Klein JD
American journal of physiology. Renal physiology 2016 Dec 1;311(6):F1189-F1197
American journal of physiology. Renal physiology 2016 Dec 1;311(6):F1189-F1197
Regulation of RCAN1 protein activity by Dyrk1A protein-mediated phosphorylation.
Jung MS, Park JH, Ryu YS, Choi SH, Yoon SH, Kwen MY, Oh JY, Song WJ, Chung SH
The Journal of biological chemistry 2011 Nov 18;286(46):40401-12
The Journal of biological chemistry 2011 Nov 18;286(46):40401-12
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human/Mouse/Rat Calcineurin B by Western Blot. Western blot shows lysates of mouse brain tissue, HT-29 human colon adenocarcinoma cell line, PC-12 rat adrenal pheochromocytoma cell line, C6 rat glioma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Human/Mouse/Rat Calcineurin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1348) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Calcineurin B at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Calcineurin B by Simple WesternTM. Simple Western lane view shows lysates of HT-29 human colon adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Calcineurin B at approximately 24 kDa (as indicated) using 2.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Calcineurin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1348). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
