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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF1083 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IFN-alpha/beta R2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse IFN-alpha/beta R2 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) IFN-gamma R2 and rmIL-20 R alpha is observed, 5% cross-reactivity with recombinant human (rh) IL-10 R beta , rmIFN-gamma RI, and rhIL-20 R beta is observed and less than 1% cross-reactivity with rhIFN-beta R alpha is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9D1R7- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Virus-Triggered ATP Release Limits Viral Replication through Facilitating IFN-β Production in a P2X7-Dependent Manner.
Differential growth of IFN-beta-engineered tumor cells in nude and IFN receptor-null mice.
Zhang C, He H, Wang L, Zhang N, Huang H, Xiong Q, Yan Y, Wu N, Ren H, Han H, Liu M, Qian M, Du B
Journal of immunology (Baltimore, Md. : 1950) 2017 Aug 15;199(4):1372-1381
Journal of immunology (Baltimore, Md. : 1950) 2017 Aug 15;199(4):1372-1381
Differential growth of IFN-beta-engineered tumor cells in nude and IFN receptor-null mice.
Zhang F, Lee J, Wang D, Dong Z
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2006 Feb;26(2):108-18
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2006 Feb;26(2):108-18
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Limitin Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN-alpha/beta R2 Antibody. Recombinant Mouse Limitin (Catalog # 1535-LM) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse Limitin (30 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IFN-alpha/beta R2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1083). The ND50 is typically 2-10 µg/mL.
