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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [23]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- V3462 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- CD3e Antibody
- Antibody type
- Monoclonal
- Description
- Purified
- Reactivity
- Human, Mouse, Rat, Bovine, Guinea Pig, Horse, Porcine, Rabbit
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- PC3/188A
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml
- Storage
- Store the CD3e antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Posttransplant Lymphoproliferative Disorders in Neuronal Xenotransplanted Macaques.
Bioengineering of injectable encapsulated aggregates of pluripotent stem cells for therapy of myocardial infarction.
CD33(+) /HLA-DR(neg) and CD33(+) /HLA-DR(+/-) Cells: Rare Populations in the Human Decidua with Characteristics of MDSC.
Peripheral Leukocyte Migration in Ferrets in Response to Infection with Seasonal Influenza Virus.
Predominance of Type 1 Innate Lymphoid Cells in the Rectal Mucosa of Patients With Non-Celiac Wheat Sensitivity: Reversal After a Wheat-Free Diet.
Supplementation of H1N1pdm09 split vaccine with heterologous tandem repeat M2e5x virus-like particles confers improved cross-protection in ferrets.
Propranolol Attenuates Surgical Stress-Induced Elevation of the Regulatory T Cell Response in Patients Undergoing Radical Mastectomy.
Immuno-histological assessment of sub-clinical acute and borderline rejection in renal allograft recipients: Data from a transplant center in India.
Expression of recipient cytomegalovirus in immunosuppressed and xenotransplanted Macaca fascicularis may be related to more severe gastrointestinal lesions.
Stem cells in the development and differentiation of the human adrenal glands.
CD43 expression in diffuse large B-cell lymphoma, not otherwise specified: CD43 is a marker of adverse prognosis.
Overexpression of human IGF-I via direct rAAV-mediated gene transfer improves the early repair of articular cartilage defects in vivo.
Huge clusters of embryonic stem cells in human embryos: a morphologic study.
Rate of atherosclerosis progression in ApoE-/- mice long after discontinuation of cola beverage drinking.
The late phase of post-stroke neurorepair in aged rats is reflected by MRI-based measures.
Direct rAAV SOX9 administration for durable articular cartilage repair with delayed terminal differentiation and hypertrophy in vivo.
CD8-alpha T-cell infiltration in human papillomavirus-related oropharyngeal carcinoma correlates with improved patient prognosis.
Cartilage constructs engineered from chondrocytes overexpressing IGF-I improve the repair of osteochondral defects in a rabbit model.
ATP-dependent potassium channel blockade strengthens microglial neuroprotection after hypoxia-ischemia in rats.
The dynamic expression of allograft inflammatory factor-1 in hepatic tissues and splenic cells of BALB/c mice with Schistosoma japonicum infection.
Tools for cellular immunology and vaccine research the in the guinea pig: monoclonal antibodies to cell surface antigens and cell lines.
Histological description of the lymphadenopathy related to Toscana virus infection. Report of a case.
Prognostic significance of survivin and caspase-3 immunohistochemical expression in patients with diffuse large B-cell lymphoma treated with rituximab and CHOP.
Cavicchioli L, Ferraresso S, Westmoreland S, Kaliyaperumal S, Knight H, Crossan C, Scobie L, Danesi A, Vadori M, Trez D, Badin RA, Hantraye P, Cozzi E
Veterinary pathology 2016 Sep 30;
Veterinary pathology 2016 Sep 30;
Bioengineering of injectable encapsulated aggregates of pluripotent stem cells for therapy of myocardial infarction.
Zhao S, Xu Z, Wang H, Reese BE, Gushchina LV, Jiang M, Agarwal P, Xu J, Zhang M, Shen R, Liu Z, Weisleder N, He X
Nature communications 2016 Oct 27;7:13306
Nature communications 2016 Oct 27;7:13306
CD33(+) /HLA-DR(neg) and CD33(+) /HLA-DR(+/-) Cells: Rare Populations in the Human Decidua with Characteristics of MDSC.
Bartmann C, Junker M, Segerer SE, Häusler SF, Krockenberger M, Kämmerer U
American journal of reproductive immunology (New York, N.Y. : 1989) 2016 May;75(5):539-56
American journal of reproductive immunology (New York, N.Y. : 1989) 2016 May;75(5):539-56
Peripheral Leukocyte Migration in Ferrets in Response to Infection with Seasonal Influenza Virus.
Music N, Reber AJ, Kim JH, York IA
PloS one 2016 Jun 17;11(6):e0157903
PloS one 2016 Jun 17;11(6):e0157903
Predominance of Type 1 Innate Lymphoid Cells in the Rectal Mucosa of Patients With Non-Celiac Wheat Sensitivity: Reversal After a Wheat-Free Diet.
Di Liberto D, Mansueto P, D'Alcamo A, Lo Pizzo M, Lo Presti E, Geraci G, Fayer F, Guggino G, Iacono G, Dieli F, Carroccio A
Clinical and translational gastroenterology 2016 Jul 7;7(7):e178
Clinical and translational gastroenterology 2016 Jul 7;7(7):e178
Supplementation of H1N1pdm09 split vaccine with heterologous tandem repeat M2e5x virus-like particles confers improved cross-protection in ferrets.
Music N, Reber AJ, Kim MC, York IA, Kang SM
Vaccine 2016 Jan 20;34(4):466-73
Vaccine 2016 Jan 20;34(4):466-73
Propranolol Attenuates Surgical Stress-Induced Elevation of the Regulatory T Cell Response in Patients Undergoing Radical Mastectomy.
Zhou L, Li Y, Li X, Chen G, Liang H, Wu Y, Tong J, Ouyang W
Journal of immunology (Baltimore, Md. : 1950) 2016 Apr 15;196(8):3460-9
Journal of immunology (Baltimore, Md. : 1950) 2016 Apr 15;196(8):3460-9
Immuno-histological assessment of sub-clinical acute and borderline rejection in renal allograft recipients: Data from a transplant center in India.
Badwal S, Kumar A, Hooda AK, Varma PP
Saudi journal of kidney diseases and transplantation : an official publication of the Saudi Center for Organ Transplantation, Saudi Arabia 2015 Nov;26(6):1232-40
Saudi journal of kidney diseases and transplantation : an official publication of the Saudi Center for Organ Transplantation, Saudi Arabia 2015 Nov;26(6):1232-40
Expression of recipient cytomegalovirus in immunosuppressed and xenotransplanted Macaca fascicularis may be related to more severe gastrointestinal lesions.
Cavicchioli L, Zanetti R, Ferraresso S, Crossan C, Barry PA, Boldrin M, Vadori M, Ferro S, Calabrese F, Scobie L, Cozzi E, Zappulli V
Xenotransplantation 2015 Mar-Apr;22(2):135-43
Xenotransplantation 2015 Mar-Apr;22(2):135-43
Stem cells in the development and differentiation of the human adrenal glands.
Terada T
Microscopy research and technique 2015 Jan;78(1):59-64
Microscopy research and technique 2015 Jan;78(1):59-64
CD43 expression in diffuse large B-cell lymphoma, not otherwise specified: CD43 is a marker of adverse prognosis.
Ma XB, Zheng Y, Yuan HP, Jiang J, Wang YP
Human pathology 2015 Apr;46(4):593-9
Human pathology 2015 Apr;46(4):593-9
Overexpression of human IGF-I via direct rAAV-mediated gene transfer improves the early repair of articular cartilage defects in vivo.
Cucchiarini M, Madry H
Gene therapy 2014 Sep;21(9):811-9
Gene therapy 2014 Sep;21(9):811-9
Huge clusters of embryonic stem cells in human embryos: a morphologic study.
Terada T
Microscopy research and technique 2014 Oct;77(10):825-31
Microscopy research and technique 2014 Oct;77(10):825-31
Rate of atherosclerosis progression in ApoE-/- mice long after discontinuation of cola beverage drinking.
Otero-Losada M, Cao G, Mc Loughlin S, Rodríguez-Granillo G, Ottaviano G, Milei J
PloS one 2014 Mar 26;9(3):e89838
PloS one 2014 Mar 26;9(3):e89838
The late phase of post-stroke neurorepair in aged rats is reflected by MRI-based measures.
Titova EM, Ghosh N, Valadez ZG, Zhang JH, Bellinger DL, Obenaus A
Neuroscience 2014 Dec 26;283:231-44
Neuroscience 2014 Dec 26;283:231-44
Direct rAAV SOX9 administration for durable articular cartilage repair with delayed terminal differentiation and hypertrophy in vivo.
Cucchiarini M, Orth P, Madry H
Journal of molecular medicine (Berlin, Germany) 2013 May;91(5):625-36
Journal of molecular medicine (Berlin, Germany) 2013 May;91(5):625-36
CD8-alpha T-cell infiltration in human papillomavirus-related oropharyngeal carcinoma correlates with improved patient prognosis.
Jung AC, Guihard S, Krugell S, Ledrappier S, Brochot A, Dalstein V, Job S, de Reynies A, Noël G, Wasylyk B, Clavel C, Abecassis J
International journal of cancer 2013 Jan 15;132(2):E26-36
International journal of cancer 2013 Jan 15;132(2):E26-36
Cartilage constructs engineered from chondrocytes overexpressing IGF-I improve the repair of osteochondral defects in a rabbit model.
Madry H, Kaul G, Zurakowski D, Vunjak-Novakovic G, Cucchiarini M
European cells & materials 2013 Apr 16;25:229-47
European cells & materials 2013 Apr 16;25:229-47
ATP-dependent potassium channel blockade strengthens microglial neuroprotection after hypoxia-ischemia in rats.
Ortega FJ, Gimeno-Bayon J, Espinosa-Parrilla JF, Carrasco JL, Batlle M, Pugliese M, Mahy N, Rodríguez MJ
Experimental neurology 2012 May;235(1):282-96
Experimental neurology 2012 May;235(1):282-96
The dynamic expression of allograft inflammatory factor-1 in hepatic tissues and splenic cells of BALB/c mice with Schistosoma japonicum infection.
Chen QR, Guan F, Yan DJ, Lei DS, Fu L, Xia HS, Zhu YH, Chen ZW, Niu AO
Tissue antigens 2012 Jan;79(1):33-41
Tissue antigens 2012 Jan;79(1):33-41
Tools for cellular immunology and vaccine research the in the guinea pig: monoclonal antibodies to cell surface antigens and cell lines.
Schäfer H, Burger R
Vaccine 2012 Aug 31;30(40):5804-11
Vaccine 2012 Aug 31;30(40):5804-11
Histological description of the lymphadenopathy related to Toscana virus infection. Report of a case.
Ranaldi R, Goteri G, Biagetti S, Cusi MG, Rossini S
Pathology, research and practice 2011 Mar 15;207(3):197-201
Pathology, research and practice 2011 Mar 15;207(3):197-201
Prognostic significance of survivin and caspase-3 immunohistochemical expression in patients with diffuse large B-cell lymphoma treated with rituximab and CHOP.
Mitrović Z, Ilić I, Aurer I, Kinda SB, Radman I, Dotlić S, Ajduković R, Labar B
Pathology oncology research : POR 2011 Jun;17(2):243-7
Pathology oncology research : POR 2011 Jun;17(2):243-7
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human Jurkat cell lysate with CD3e antibody (clone PC3/188A). Expected molecular weight ~23 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of human tonsil tissue with CD3e antibody (clone PC3/188A). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free CD3e Antibody (clone PC3/188A). Confirmation of Integrity and Purity of the Antibody.
