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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AM12005PU-N - Provider product page
- Provider
- OriGene
- Product name
- CD3E (activation epitope) mouse monoclonal antibody, clone APA1/1, Aff - Purified
- Antibody type
- Monoclonal
- Description
- CD3E (activation epitope) mouse monoclonal antibody, clone APA1/1, Aff - Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CD3E
- Isotype
- IgG
- Antibody clone number
- APA1/1
- Vial size
- 100 µg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Jurkat cell lysate was probed with anti-CD3 epsilon? (0.5ug/ml).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- T-cell Activation by an Antigen presenting Cell. Panel A is an image of a single Antigen-Presenting Cell (APC; a Raji cell, stained blue with CMAC dye) activated by Staphylococcal enterotoxin E in contact with a single T-cell (a Jurkat cell). Panel B is an image of the two cells, stained with an anti-CD3?? antiserum (a 1:3,000 dilution) and anti-CD3??, clone APA1/1 ( #; 10ug/ml). Visualization of CD3?? or CD3?? was with an anti-rabbit Alexa 488 (green) or anti-mouse Alexa 594 (red) conjugate, respectively; the yellow color is produced by co-localization of CD3?? and CD3?? (clone APA1/1) staining. Data generously provided by Dr. Balbino Alarcon, Centro de Biologia Molecular, CSIC, Madrid, Spain.
- Validation comment
- IF
