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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NBP2-76424 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Monoclonal CD3 epsilon Antibody
- Antibody type
- Monoclonal
- Description
- Antigen Affinity-purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 2 - 8 C 1 year from date of receipt
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424] - Detection of human CD3E by WB of Jurkat, MOLT-4, K-562, SK-MEL-28 (SK), and HeLa lysate Antibody: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (NBP2-76424). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-Actin.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424] - Detection of human CD3E by WB of immunoprecipitates from Jurkat lysate. Antibodies: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (NBP2-76424).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424] - Detection of human CD3E in FFPE tonsil by IHC. Antibody: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (NBP2-76424). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424] - Detection of human CD3E in Jurkat cells by flow cytometry. Antibody: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (NBP2-76424) (red shaded) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
