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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- SM1754P - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#SM1754P, RRID:AB_975696
- Product name
- anti CD3 (Cytopl. Dom.)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide sequence derived from Cytoplasmic epitope of CD3
- Reactivity
- Human, Mouse, Canine, Chicken/Avian, Porcine
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- CD3-12
- Vial size
- 0.2 mg
- Concentration
- 1.0 mg/ml
Submitted references Chemotherapy induces intratumoral expression of chemokines in cutaneous melanoma, favoring T-cell infiltration and tumor control.
B cell-derived vascular endothelial growth factor A promotes lymphangiogenesis and high endothelial venule expansion in lymph nodes.
Hong M, Puaux AL, Huang C, Loumagne L, Tow C, Mackay C, Kato M, Prévost-Blondel A, Avril MF, Nardin A, Abastado JP
Cancer research 2011 Nov 15;71(22):6997-7009
Cancer research 2011 Nov 15;71(22):6997-7009
B cell-derived vascular endothelial growth factor A promotes lymphangiogenesis and high endothelial venule expansion in lymph nodes.
Shrestha B, Hashiguchi T, Ito T, Miura N, Takenouchi K, Oyama Y, Kawahara K, Tancharoen S, Ki-I Y, Arimura N, Yoshinaga N, Noma S, Shrestha C, Nitanda T, Kitajima S, Arimura K, Sato M, Sakamoto T, Maruyama I
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):4819-26
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):4819-26
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Jurkat human acute T-cell leukemia whole cell lysate probed with with anti Human CD3 antibody Cat.-No SM1754P. followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemitry in Formalin-fixed, Paraffin embedded human tonsil stained with CD3 antibody Cat.-No SM1754P following heat mediated antigen retrieval in EDTA buffer pH 9.0
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Staining of Human peripheral blood lymphocytes (permeabilised) with Rat anti Human CD3 antibody Cat.-No SM1754P.
