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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 600-401-A69 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-A69, RRID:AB_2240594
- Product name
- Anti-TLR9 (RABBIT) Antibody - 600-401-A69
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of Rabbit Anti-TLR-9 Antibody. Lane 1: Jurkat whole cell lysate using 0.5 µg/ml. Lane 2: Jurkat whole cell lysate using 1.0 µg/ml. Lane 3: Jurkat whole cell lysate using 2.0 µg/ml. Load: 10 µg per lane. Primary antibody: TLR 9 antibody overnight at 4°C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 116 kDa, ~94 kDa for TLR9. Other band(s): none.
- Validation comment
- Western Blot
