SM7105P

antibody from Acris Antibodies GmbH

Targeting: TLR9 CD289

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Immunoelectron microscopy (Recommended by provider)

Gel shift (Recommended by provider)

Antibody Data

Product number

SM7105P

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#SM7105P, RRID:AB_1008081

Product name

anti CD289 / TLR9 (268-284)

Antibody type

Monoclonal

Antigen

KLH-conjugated synthetic peptide corresponding to amino acids 268-284 of Human TRL9 isoform A

Reactivity

Human, Mouse, Rat, Canine, Simian

Host

Mouse

Isotype

IgG

Antibody clone number

26C593.2

Vial size

0.1 mg

Concentration

0.5 mg/ml

References

Submitted references

Expression of TLR9 in tumor-infiltrating mononuclear cells enhances angiogenesis and is associated with a worse survival in lung cancer.

Belmont L, Rabbe N, Antoine M, Cathelin D, Guignabert C, Kurie J, Cadranel J, Wislez M
International journal of cancer. Journal international du cancer 2014 Feb 15;134(4):765-77

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Expression of Toll-like receptor-9 is associated with poor progression-free survival in prostate cancer.

Väisänen MR, Jukkola-Vuorinen A, Vuopala KS, Selander KS, Vaarala MH
Oncology letters 2013 May;5(5):1659-1663

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Expression of Toll-like receptor 9 in mouse and human lungs.

Schneberger D, Caldwell S, Kanthan R, Singh B
Journal of anatomy 2013 May;222(5):495-503

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Expression of Toll-like receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum.

Figueiredo MM, Amorim IF, Pinto AJ, Barbosa VS, Pinheiro Lde J, Deoti B, Faria AM, Tafuri WL
BMC immunology 2013 May 14;14:22

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Short DNA sequences and bacterial DNA induce esophageal, gastric, and colorectal cancer cell invasion.

Kauppila JH, Karttunen TJ, Saarnio J, Nyberg P, Salo T, Graves DE, Lehenkari PP, Selander KS
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2013 Jun;121(6):511-22

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Distinct autoantibody profiles in systemic lupus erythematosus patients are selectively associated with TLR7 and TLR9 upregulation.

Chauhan SK, Singh VV, Rai R, Rai M, Rai G
Journal of clinical immunology 2013 Jul;33(5):954-64

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Spontaneous production of immunoglobulin M in human epithelial cancer cells.

Hu F, Zhang L, Zheng J, Zhao L, Huang J, Shao W, Liao Q, Ma T, Geng L, Yin CC, Qiu X
PloS one 2012;7(12):e51423

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TLR4 and TLR9 are induced in oral lichen planus.

Siponen M, Kauppila JH, Soini Y, Salo T
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 2012 Nov;41(10):741-7

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The circadian clock controls toll-like receptor 9-mediated innate and adaptive immunity.

Silver AC, Arjona A, Walker WE, Fikrig E
Immunity 2012 Feb 24;36(2):251-61

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Campylobacter jejuni disrupts protective Toll-like receptor 9 signaling in colonic epithelial cells and increases the severity of dextran sulfate sodium-induced colitis in mice.

O'Hara JR, Feener TD, Fischer CD, Buret AG
Infection and immunity 2012 Apr;80(4):1563-71

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Molecular chaperoning by glucose-regulated protein 170 in the extracellular milieu promotes macrophage-mediated pathogen sensing and innate immunity.

Zuo D, Yu X, Guo C, Yi H, Chen X, Conrad DH, Guo TL, Chen Z, Fisher PB, Subjeck JR, Wang XY
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2012 Apr;26(4):1493-505

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Human papillomavirus deregulates the response of a cellular network comprising of chemotactic and proinflammatory genes.

Karim R, Meyers C, Backendorf C, Ludigs K, Offringa R, van Ommen GJ, Melief CJ, van der Burg SH, Boer JM
PloS one 2011 Mar 14;6(3):e17848

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Expression of toll-like receptor 9 in lungs of pigs, dogs and cattle.

Schneberger D, Lewis D, Caldwell S, Singh B
International journal of experimental pathology 2011 Feb;92(1):1-7

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Immunolocalization of pulmonary intravascular macrophages, TLR4, TLR9 and IL-8 in normal and Pasteurella multocida-infected lungs of water buffalo (Bubalus bubalis).

Sethi RS, Brar RS, Singh O, Singh B
Journal of comparative pathology 2011 Feb-Apr;144(2-3):135-44

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EBV lytic-phase protein BGLF5 contributes to TLR9 downregulation during productive infection.

van Gent M, Griffin BD, Berkhoff EG, van Leeuwen D, Boer IG, Buisson M, Hartgers FC, Burmeister WP, Wiertz EJ, Ressing ME
Journal of immunology (Baltimore, Md. : 1950) 2011 Feb 1;186(3):1694-702

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TLR9 signaling failure renders Peyer's patch regulatory B cells unresponsive to stimulation with CpG oligodeoxynucleotides.

Booth JS, Arsenault R, Napper S, Griebel PJ, Potter AA, Babiuk LA, Mutwiri GK
Journal of innate immunity 2010;2(5):483-94

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Nasolacrimal duct closure modulates ocular mucosal and systemic CD4(+) T-cell responses induced following topical ocular or intranasal immunization.

Chentoufi AA, Dasgupta G, Nesburn AB, Bettahi I, Binder NR, Choudhury ZS, Chamberlain WD, Wechsler SL, BenMohamed L
Clinical and vaccine immunology : CVI 2010 Mar;17(3):342-53

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Toll-like receptor-induced innate immune responses in non-parenchymal liver cells are cell type-specific.

Wu J, Meng Z, Jiang M, Zhang E, Trippler M, Broering R, Bucchi A, Krux F, Dittmer U, Yang D, Roggendorf M, Gerken G, Lu M, Schlaak JF
Immunology 2010 Mar;129(3):363-74

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Protein kinase D1 is essential for the proinflammatory response induced by hypersensitivity pneumonitis-causing thermophilic actinomycetes Saccharopolyspora rectivirgula.

Kim YI, Park JE, Brand DD, Fitzpatrick EA, Yi AK
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 15;184(6):3145-56

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Expression of Toll-like receptor 9 in renal podocytes in childhood-onset active and inactive lupus nephritis.

Machida H, Ito S, Hirose T, Takeshita F, Oshiro H, Nakamura T, Mori M, Inayama Y, Yan K, Kobayashi N, Yokota S
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2010 Aug;25(8):2530-537

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TLR9 expression in glioma tissues correlated to glioma progression and the prognosis of GBM patients.

Wang C, Cao S, Yan Y, Ying Q, Jiang T, Xu K, Wu A
BMC cancer 2010 Aug 10;10:415

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Toll-like receptor-9 expression is inversely correlated with estrogen receptor status in breast cancer.

Jukkola-Vuorinen A, Rahko E, Vuopala KS, Desmond R, Lehenkari PP, Harris KW, Selander KS
Journal of innate immunity 2009;1(1):59-68

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Toll-like receptor expression in normal ovary and ovarian tumors.

Zhou M, McFarland-Mancini MM, Funk HM, Husseinzadeh N, Mounajjed T, Drew AF
Cancer immunology, immunotherapy : CII 2009 Sep;58(9):1375-85

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TLR9 is expressed in idiopathic interstitial pneumonia and its activation promotes in vitro myofibroblast differentiation.

Meneghin A, Choi ES, Evanoff HL, Kunkel SL, Martinez FJ, Flaherty KR, Toews GB, Hogaboam CM
Histochemistry and cell biology 2008 Nov;130(5):979-92

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Identification of two subpopulations of purified human blood B cells, CD27- CD23+ and CD27high CD80+, that strongly express cell surface Toll-like receptor 9 and secrete high levels of interleukin-6.

Cognasse F, Hamzeh-Cognasse H, Lafarge S, Chavarin P, Pozzetto B, Richard Y, Garraud O
Immunology 2008 Nov;125(3):430-7

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Expression of TLR9 within human glioblastoma.

Meng Y, Kujas M, Marie Y, Paris S, Thillet J, Delattre JY, Carpentier AF
Journal of neuro-oncology 2008 May;88(1):19-25

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Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry.

Burgener IA, Jungi TW
Veterinary immunology and immunopathology 2008 Jul 15;124(1-2):184-91

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Localization of Toll-like receptors on epididymal epithelial cells and spermatozoa.

Palladino MA, Savarese MA, Chapman JL, Dughi MK, Plaska D
American journal of reproductive immunology (New York, N.Y. : 1989) 2008 Dec;60(6):541-55

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Surface expression of Toll-like receptor 9 is upregulated on intestinal epithelial cells in response to pathogenic bacterial DNA.

Ewaschuk JB, Backer JL, Churchill TA, Obermeier F, Krause DO, Madsen KL
Infection and immunity 2007 May;75(5):2572-9

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Nucleic acids exert a sequence-independent cooperative effect on sequence-dependent activation of Toll-like receptor 9.

Kindrachuk J, Potter JE, Brownlie R, Ficzycz AD, Griebel PJ, Mookherjee N, Mutwiri GK, Babiuk LA, Napper S
The Journal of biological chemistry 2007 May 11;282(19):13944-53

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Members of the Toll-like receptor family of innate immunity pattern-recognition receptors are abundant in the male rat reproductive tract.

Palladino MA, Johnson TA, Gupta R, Chapman JL, Ojha P
Biology of reproduction 2007 Jun;76(6):958-64

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Toll-like receptor 9 expression in murine and human adrenal glands and possible implications during inflammation.

Tran N, Koch A, Berkels R, Boehm O, Zacharowski PA, Baumgarten G, Knuefermann P, Schott M, Kanczkowski W, Bornstein SR, Lightman SL, Zacharowski K
The Journal of clinical endocrinology and metabolism 2007 Jul;92(7):2773-83

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Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.

Wong CK, Cheung PF, Ip WK, Lam CW
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96

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TGF-alpha regulates TLR expression and function on epidermal keratinocytes.

Miller LS, Sørensen OE, Liu PT, Jalian HR, Eshtiaghpour D, Behmanesh BE, Chung W, Starner TD, Kim J, Sieling PA, Ganz T, Modlin RL
Journal of immunology (Baltimore, Md. : 1950) 2005 May 15;174(10):6137-43

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis of TLR9 in A) human PBMC, B) human intestine, C) mouse intestine, and D) rat intestine tissue lysates using the antibody at a dilution of 3 µg/ml. Lane E shows antibody tested at 5 ug/ml in human MCF7 cell lysate.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis of TLR9 antibody in A) human PBMCsB) human intestine C) mouse intestine D) rat intestine tissue lysates using SM7105P at 3 µg/ml and goat anti-mouse HRP conjugate antibody as secondary.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

IHC analysis of TLR9 in Adenocarcinoma of the lung (A), A549 cells (C), and malignant lung tissues (F) at 1:100 dilution, (600x magnification).

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Lung: Formalin-Fixed Paraffin-Embedded (FFPE)

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human spleen probed with TLR9 antibody (Cat.-No.: SM7105P) at 5 ug/ml. Human tissue TMA was used for this test.Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Flow cytometry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Intracellular flow analysis of TLR9 in Ramos cells using 0.1 ug of SM7105P. Shaded histogram represents Ramos cells without antibody. Green represents Isotype Control, Red represents anti-TLR9 antibody. FITC conjugated Goat anti-Mouse IgG was used.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Intracellular flow analysis of TLR9 in human PBMC using 0.5 µg of TLR9 antibody (red) and isotype control antibody (green). (Cat.-No.: SM7105P)