66133-1-IG

antibody from Invitrogen Antibodies

Targeting: AMY2B AMY2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

66133-1-IG

Provider

Invitrogen Antibodies

Product name

Amylase alpha Monoclonal Antibody (2F1G8)

Antibody type

Monoclonal

Antigen

Other

Description

This antibody can recognize amylase alpha.

Antibody clone number

2F1G8

Concentration

0.79 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

0.6 µg rat pancreas tissue lysate were subjected to SDS PAGE followed by western blot with 66133-1-Ig (Amylase, alpha antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human saliva were subjected to SDS PAGE followed by western blot with 66133-1-IG (Amylase, alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of BxPC-3 cells using 66133-1-IG (Amylase, alpha antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of BxPC-3 cells using 66133-1-IG (Amylase, alpha antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 66133-1-IG ( Amylase, alpha antibody at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 66133-1-IG ( Amylase, alpha antibody at dilution of 1:50 (under 40x lens).