DMABT-45616MH

antibody from Creative Diagnostics

Targeting: FUT4 CD15, ELFT, FCT3A, FUC-TIV

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

DMABT-45616MH

Provider

Creative Diagnostics

Proper citation

Creative Diagnostics Cat#DMABT-45616MH, RRID:AB_2394205

Product name

Anti-CD15 monoclonal antibody, clone Cv39

Antibody type

Monoclonal

Description

CD15 (3-fucosyl-N-acetyl-lactosamine) is a cluster of differentiation antigen - an immunologically significant molecule. CD15 is a carbohydrate adhesion molecule (not a protein) that can be expressed on glycoproteins, glycolipids and proteoglycans. CD15 is expressed on Reed-Sternberg cells of Hodgkin's disease and by various other cell types including myeloid cells and epithelial cells. Antibodies to CD15 recognize a pentasaccharide sequence occurring in lacto-N-fucopentaose III ceramide (also referred to as X hapten of Lex) found in higher glycolipids and glycoproteins. A review by Arber et al. has reported that antibodies to CD15 demonstrate positive staining in 87% of Hodgkin's disease including nodular sclerosing, mixed cellularity, and lymphocyte depletion, whereas the lymphocyte predominant variant exhibits a lower rate of positivity (37%). Among non-Hodgkin's lymphoma, 13% express CD15 including 4.1% B-cell, 21% T-cell, and 17% null-cell. CD15 expression has also been demonstrated in acute myeloid leukemia (65%) and chronic myelogenous leukemia (96% chronic phase and 54% blast phase). A relatively low level of CD15 expression has been reported in acute lymphoblastic leukemia (5.7% overall) with positivity observed in 7.7% common or precursor B-cell, 0% B-cell, 7.7% T-cell and 17.3% nullcell. Carcinomas derived from various organs have also been shown to be CD15 positive (56%) including adenocarcinomas, squamous cell carcinomas and undifferentiated large and small cell carcinomas.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgM

Antibody clone number

Cv39

Vial size

200 _g

Concentration

Ig concentration 1.0mg/ml

Storage

Store at +4 oC or at -20 oC if preferred.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.