NB100-1831

antibody from Novus Biologicals

Targeting: FUT4 CD15, ELFT, FCT3A, FUC-TIV

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-1831

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-1831, RRID:AB_10002130

Product name

Mouse Monoclonal SSEA-1 Antibody

Antibody type

Monoclonal

Description

IgM purified.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgM

Vial size

0.1 ml

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Reprogramming of chimpanzee fibroblasts into a multipotent cancerous but not fully pluripotent state by transducing iPSC factors in 2i/LIF culture.

Lin ZY, Nakai R, Hirai H, Kozuka D, Katayama S, Nakamura SI, Okada S, Kitajima R, Imai H, Okano H, Imamura M
Differentiation; research in biological diversity 2020 Mar - Apr;112:67-76

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Derivation of novel naive-like porcine embryonic stem cells by a reprogramming factor-assisted strategy.

Zhang M, Wang C, Jiang H, Liu M, Yang N, Zhao L, Hou D, Jin Y, Chen Q, Chen Y, Wang J, Dai Y, Li R
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Aug;33(8):9350-9361

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Rac1 contributes to cerebral ischemia reperfusion-induced injury in mice by regulation of Notch2.

Meng S, Su Z, Liu Z, Wang N, Wang Z
Neuroscience 2015 Oct 15;306:100-14

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Specific knockdown of Oct4 and beta2-microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells.

Matin MM, Walsh JR, Gokhale PJ, Draper JS, Bahrami AR, Morton I, Moore HD, Andrews PW
Stem cells (Dayton, Ohio) 2004;22(5):659-68

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Long-term proliferation in culture and germline transmission of mouse male germline stem cells.

Kanatsu-Shinohara M, Ogonuki N, Inoue K, Miki H, Ogura A, Toyokuni S, Shinohara T
Biology of reproduction 2003 Aug;69(2):612-6

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Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens.

Henderson JK, Draper JS, Baillie HS, Fishel S, Thomson JA, Moore H, Andrews PW
Stem cells (Dayton, Ohio) 2002;20(4):329-37

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Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro.

Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A
Nature biotechnology 2000 Apr;18(4):399-404

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: SSEA-1 Antibody (MC-480) [NB100-1831] - Simple Western lane view shows a specific band for SSEA1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: SSEA-1 Antibody (MC-480) [NB100-1831] - An intracellular stain was performed on Jurkat cells with SSEA-1 Antibody (MC-480)NB100-1831 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse IgM Alexa Fluor 488-conjugated secondary antibody.