H00007290-B01P

antibody from Abnova Corporation

Targeting: HIRA DGCR1, TUP1, TUPLE1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007290-B01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007290-B01P, RRID:AB_1576008

Product name

HIRA purified MaxPab mouse polyclonal antibody (B01P)

Antibody type

Polyclonal

Description

Mouse polyclonal antibody raised against a full-length human HIRA protein.

Antigen sequence

MKLLKPTWVNHNGKPIFSVDIHPDGTKFATGGQGQ
DSGKVVIWNMSPVLQEDDEKDENIPKMLCQMDNHL
ACVNCVRWSNSGMYLASGGDDKLIMVWKRATYIGP
STVFGSSGKLANVEQWRCVSILRNHSGDVMDVAWS
PHDAWLASCSVDNTVVIWNAVKFPEILATLRGHSG
LVKGLTWDPVGKYIASQADDRSLKVWRTLDWQLET
SITKPFDECGGTTHVLRLSWSPDGHYLVSAHAMNN
SGPTAQIIEREGWKTNMDFVGHRKAVTVVKFNPKI
FKKKQKNGSSAKPSCPYCCCAVGSKDRSLSVWLTC
LKRPLVVIHELFDKSIMDISWTLNGLGILVCSMDG
SVAFLDFSQDELGDPLSEEEKSRIHQSTYGKSLAI
MTEAQLSTAVIENPEMLKYQRRQQQQQLDQKSAAT
REMGSATSVAGVVNGESLEDIRKNLLKKQVETRTA
DGRRRITPLCIAQLDTGDFSTAFFNSIPLSGSLAG
TMLSSHSSPQLLPLDSSTPNSFGASKPCTEPVVAA
SARPAGDSVNKDSMNATSTPAALSPSVLTTPSKIE
PMKAFDSRFTERSKATPGAPALTSMTPTAVERLKE
QNLVKELRPRDLLESSSDSDEKVPLAKASSLSKRK
LELEVETVEKKKKGRPRKDSRLMPVSLSVQSPAAL
TAEKEAMCLSAPALALKLPIPSPQRAFTLQVSSDP
SMYIEVENEVTVVGGVKLSRLKCNREGKEWETVLT
SRILTAAGSCDVVCVACEKRMLSVFSTCGRRLLSP
ILLPSPISTLHCTGSYVMALTAAATLSVWDVHRQV
VVVKEESLHSILAGSDMTVSQILLTQHGIPVMNLS
DGKAYCFNPSLSTWNLVSDKQDSLAQCADFRSSLP
SQDAMLCSGPLAIIQGRTSNSGRQAARLFSVPHVV
QQETTLAYLENQVAAALTLQSSHEYRHWLLVYARY
LVNEGFEYRLREICKDLLGPVHYSTGSQWESTVVG
LRKRELLKELLPVIGQNLRFQRLFTECQEQLDILR
DK


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

HP1-mediated formation of alternative lengthening of telomeres-associated PML bodies requires HIRA but not ASF1a.

Jiang WQ, Nguyen A, Cao Y, Chang AC, Reddel RR
PloS one 2011 Feb 15;6(2):e17036

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of HIRA expression in transfected 293T cell line (H00007290-T01) by HIRA MaxPab polyclonal antibody.Lane 1: HIRA transfected lysate(111.87 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to HIRA on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol