Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP8088b - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP8088b, RRID:AB_2161856
- Product name
- PACSIN2 Antibody (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- PACSIN2 Antibody (S357) (Cat.# AP8088b) western blot analysis in Daudi cell line and mouse brain lysates (35ug/lane).This demonstrates the PACSIN2 antibody detected the PACSIN2 protein (arrow).
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "PACSIN2 Antibody (S357) (Cat.# AP8088b) western blot analysis in Daudi,HL-60 cell line and mouse brain,rat heart lysates (35ug/lane).This demonstrates the PACSIN2 antibody detected the PACSIN2 protein (arrow)."
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Immunofluorescent analysis of Hela cells, using PACSIN2 Antibody (C-term) (Cat. #AP8088b). AP8088b was diluted at 1:100 dilution. Alexa Fluor® 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red)."
- Primary Ab dilution
- 1:100
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."
- Primary Ab dilution
- 1:50~100