Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- V7618 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- CD20 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G affinity chromatography
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml, 1 mg/ml (BSA/azide free format), Prediluted (IHC only format)
- Storage
- CD20 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human Raji lysate with CD20 antibody (clone MS4A1/3411). Predicted molecular weight ~33 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescence staining of human Raji cells with CD20 antibody (green, clone MS4A1/3411) and Reddot nuclear stain (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human tonsil tissue with CD20 antibody (clone MS4A1/3411). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human Raji cells with CD20 antibody (clone MS4A1/3411); Red=isotype control, Blue= CD20 antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free CD20 antibody (clone MS4A1/3411) as confirmation of integrity and purity.