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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-85198 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CSB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution.
- Concentration
- 0.69 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CSB in A) 293T whole cell lysate/extract, B) A431 whole cell lysate/extract, C) HeLa whole cell lysate/extract, D) HepG2 whole cell lysate/extract using CSB polyclonal antibody (Product # PA5-85198) using 30 µg of sample at a dilution of 1:500. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CSB was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CSB Polyclonal Antibody (Product # PA5-85198) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of CSB in 4% paraformaldehyde-fixed HeLa cells using CSB polyclonal antibody (Product # PA5-85198) at a dilution of 1:500. Sample was then incubated with phalloidin and Hoechst secondary antibody at a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CSB in paraffin-embedded rat hind brain using CSB polyclonal antibody (Product # PA5-85198) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CSB in paraffin-embedded mouse muscle using CSB polyclonal antibody (Product # PA5-85198) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of CSB in 293T whole cell extracts with CSB polyclonal antibody (Product # PA5-85198) using 5 µg of sample, followed by anti-Rabbit IgG secondary antibody.
