M00356-1

antibody from Boster Biological Technology

Targeting: CEACAM5 CD66e, CEA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

M00356-1

Provider

Boster Biological Technology

Product name

Anti-CEA (CD66e) CEACAM5 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for CD66e/CEACAM5 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF, IHC, ICC, WB. Reactive species: Human CD66e/CEACAM5 information: Molecular Weight: 76795 MW; Subcellular Localization: Cell membrane ; Lipid-anchor, GPI-anchor ; Tissue Specificity: Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.

Reactivity

Human

Host

Rabbit

Antibody clone number

AAOA-3

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of CEA(CD66e) expression in Human colon cancer lysate (M00356-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEACAM5 monoclonal antibody (Catalog # M00356-1) overnight at 4

Additional image