MA5-13714

antibody from Invitrogen Antibodies

Targeting: CEACAM5 CD66e, CEA

Provider product page for MA5-13714

Western blot

Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [21]
Comments [0]
Validations
Immunocytochemistry [1]
Immunohistochemistry [1]
Flow cytometry [2]
Other assay [1]

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Product number: MA5-13714 - Provider product page
Provider: Invitrogen Antibodies
Product name: Anti-CEA Monoclonal Antibody (COL-1)
Antibody type: Monoclonal
Antigen: Other
Description: MA5-13720 targets Cdk4 in IF, IP, and WB applications and shows reactivity with Human, mouse, and Rat samples. The MA5-13720 immunogen is purified recombinant cdk4 protein.
Reactivity: Human, Mouse, Rat
Host: Mouse
Isotype: IgG
Antibody clone number: COL-1
Vial size: 500 µL
Concentration: 0.2 mg/ml
Storage: 4° C

CEACAM5 protein structure - MA5-13714 shown in red.

Submitted references Uropathogenic E. coli Exploit CEA to Promote Colonization of the Urogenital Tract Mucosa.

Muenzner P, Kengmo Tchoupa A, Klauser B, Brunner T, Putze J, Dobrindt U, Hauck CR
PLoS pathogens 2016 May;12(5):e1005608

Comparative expression patterns and diagnostic efficacies of SR splicing factors and HNRNPA1 in gastric and colorectal cancer.

Park WC, Kim HR, Kang DB, Ryu JS, Choi KH, Lee GO, Yun KJ, Kim KY, Park R, Yoon KH, Cho JH, Lee YJ, Chae SC, Park MC, Park DS
BMC cancer 2016 Jun 10;16:358

Solid-pseudopapillary neoplasm of the pancreas: Clinicopathologic and immunohistochemical analysis of nine cases.

Ozguven BY, Tuncel D, Polat N, Sakiz D, Kabukcuoglu F, Koksal H, Kaya H
Indian journal of pathology & microbiology 2015 Jul-Sep;58(3):292-5

Global analysis of neutrophil responses to Neisseria gonorrhoeae reveals a self-propagating inflammatory program.

Sintsova A, Sarantis H, Islam EA, Sun CX, Amin M, Chan CH, Stanners CP, Glogauer M, Gray-Owen SD
PLoS pathogens 2014 Sep;10(9):e1004341

Arginase II expressed in cancer-associated fibroblasts indicates tissue hypoxia and predicts poor outcome in patients with pancreatic cancer.

Ino Y, Yamazaki-Itoh R, Oguro S, Shimada K, Kosuge T, Zavada J, Kanai Y, Hiraoka N
PloS one 2013;8(2):e55146

Multiple breast cancer cell-lines derived from a single tumor differ in their molecular characteristics and tumorigenic potential.

Mosoyan G, Nagi C, Marukian S, Teixeira A, Simonian A, Resnick-Silverman L, DiFeo A, Johnston D, Reynolds SR, Roses DF, Mosoian A
PloS one 2013;8(1):e55145

Sinonasal tract mucoepidermoid carcinoma: a clinicopathologic and immunophenotypic study of 19 cases combined with a comprehensive review of the literature.

Wolfish EB, Nelson BL, Thompson LD
Head and neck pathology 2012 Jun;6(2):191-207

Primary malignant deciduoid peritoneal mesothelioma: a report of the cytohistological and immunohistochemical appearances.

Ustun H, Astarci HM, Sungu N, Ozdemir A, Ekinci C
Diagnostic cytopathology 2011 Jun;39(6):402-8

Low-grade mucinous adenocarcinoma of the uterine corpus: a rare and deceptively bland form of endometrial carcinoma.

Fujiwara M, Longacre TA
The American journal of surgical pathology 2011 Apr;35(4):537-44

In vitro constitution and in vivo implantation of engineered skin constructs with sweat glands.

Huang S, Xu Y, Wu C, Sha D, Fu X
Biomaterials 2010 Jul;31(21):5520-5

Molecular events associated with epithelial to mesenchymal transition of nasopharyngeal carcinoma cells in the absence of Epstein-Barr virus genome.

Lin JC, Liao SK, Lee EH, Hung MS, Sayion Y, Chen HC, Kang CC, Huang LS, Cherng JM
Journal of biomedical science 2009 Nov 24;16:105

A genetically engineered adenovirus vector targeted to CD40 mediates transduction of canine dendritic cells and promotes antigen-specific immune responses in vivo.

Thacker EE, Nakayama M, Smith BF, Bird RC, Muminova Z, Strong TV, Timares L, Korokhov N, O'Neill AM, de Gruijl TD, Glasgow JN, Tani K, Curiel DT
Vaccine 2009 Nov 23;27(50):7116-24

Antigen expression of human eccrine sweat glands.

Li HH, Zhou G, Fu XB, Zhang L
Journal of cutaneous pathology 2009 Mar;36(3):318-24

Function of CSE1L/CAS in the secretion of HT-29 human colorectal cells and its expression in human colon.

Tsao TY, Tsai CS, Tung JN, Chen SL, Yue CH, Liao CF, Wang CC, Jiang MC
Molecular and cellular biochemistry 2009 Jul;327(1-2):163-70

Evaluation of the value of frozen tissue section used as "gold standard" for immunohistochemistry.

Shi SR, Liu C, Pootrakul L, Tang L, Young A, Chen R, Cote RJ, Taylor CR
American journal of clinical pathology 2008 Mar;129(3):358-66

Sebaceous carcinoma of the eyelids: thirty cases from Japan.

Izumi M, Mukai K, Nagai T, Matsubayashi J, Iwaya K, Chiu CS, Goto H
Pathology international 2008 Aug;58(8):483-8

Oral premalignant lesions induce immune reactivity to both premalignant oral lesions and head and neck squamous cell carcinoma.

Young MR, Neville BW, Chi AC, Lathers DM, Boyd Gillespie M, Day TA
Cancer immunology, immunotherapy : CII 2007 Jul;56(7):1077-86

Detection of micrometastases and skip metastases with ex vivo sentinel node mapping in carcinoma of the colon and rectum.

Yagci G, Unlu A, Kurt B, Can MF, Kaymakcioglu N, Cetiner S, Tufan T, Sen D
International journal of colorectal disease 2007 Feb;22(2):167-73

Mesenchymal hamartoma of the liver in adulthood: immunohistochemical profiles, clinical and histopathological features in two patients.

Yesim G, Gupse T, Zafer U, Ahmet A
Journal of hepato-biliary-pancreatic surgery 2005;12(6):502-7

Heightened susceptibility to chronic gastritis, hyperplasia and metaplasia in Kcnq1 mutant mice.

Elso CM, Lu X, Culiat CT, Rutledge JC, Cacheiro NL, Generoso WM, Stubbs LJ
Human molecular genetics 2004 Nov 15;13(22):2813-21

Secretory meningiomas.

Colakoğlu N, Demirtaş E, Oktar N, Yüntem N, Islekel S, Ozdamar N
Journal of neuro-oncology 2003 May;62(3):233-41

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of CD66e (green) showing staining in the cytoplasm and membrane of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD66e monoclonal antibody (Product # MA5-13714) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Formalin-fixed, paraffin-embedded human colon carcinoma stained with CEA antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic and luminal surface staining of tumor cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of CD66e in PC12 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD66e monoclonal antibody (Product # MA5-13714) at a dilution of 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of CD66e in blood granulocytes compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and primary antibody (Product # MA5-13714) at a dilution of 2 ug/test. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4ºC in the dark. FACS analysis was performed using 400 ul of cell buffer.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunoprecipitation of CD66e using CD66e Monoclonal Antibody (Product # MA5-13714) on denatured Human LS174T Cells.