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Western blot
Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [11]
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- Validations
- Immunocytochemistry [1]
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- Product number
- ALX-805-080-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-805-080-C100, RRID:AB_2052159
- Product name
- pan-CEACAM (1,3,4,5,6) (human) monoclonal antibody (D14HD11)
- Antibody type
- Monoclonal
- Antigen
- Purified from natural sources
- Description
- Protein G-affinity purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- D14HD11
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles.
Submitted references The tumorigenic potential of human CX-1 colon adenocarcinoma cells depends on carcinoembryonic antigen (CEACAM5) expression.
Neisserial binding to CEACAM1 arrests the activation and proliferation of CD4+ T lymphocytes.
Distinct mechanisms of internalization of Neisseria gonorrhoeae by members of the CEACAM receptor family involving Rac1- and Cdc42-dependent and -independent pathways.
Homophilic adhesion of human CEACAM1 involves N-terminal domain interactions: structural analysis of the binding site.
Pathogenic Neisseria trigger expression of their carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1; previously CD66a) receptor on primary endothelial cells by activating the immediate early response transcription factor, nuclear factor-kappaB.
Carcinoembryonic antigen family receptor specificity of Neisseria meningitidis Opa variants influences adherence to and invasion of proinflammatory cytokine-activated endothelial cells.
Recruitment of CD55 and CD66e brush border-associated glycosylphosphatidylinositol-anchored proteins by members of the Afa/Dr diffusely adhering family of Escherichia coli that infect the human polarized intestinal Caco-2/TC7 cells.
Molecular analysis of neisserial Opa protein interactions with the CEA family of receptors: identification of determinants contributing to the differential specificities of binding.
CEA-related proteins on human urothelial cell lines of different transformation grades.
CD66 carcinoembryonic antigens mediate interactions between Opa-expressing Neisseria gonorrhoeae and human polymorphonuclear phagocytes.
Production and characterization of monoclonal antibodies against carcinoembryonic antigen (CEA).
Baczyńska D, Wietrzyk J, Madej J, Krop-Watorek A, Dabrowska A, Widerak K, Opolski A, Ugorski M
Cellular & molecular biology letters 2003;8(2):471-86
Cellular & molecular biology letters 2003;8(2):471-86
Neisserial binding to CEACAM1 arrests the activation and proliferation of CD4+ T lymphocytes.
Boulton IC, Gray-Owen SD
Nature immunology 2002 Mar;3(3):229-36
Nature immunology 2002 Mar;3(3):229-36
Distinct mechanisms of internalization of Neisseria gonorrhoeae by members of the CEACAM receptor family involving Rac1- and Cdc42-dependent and -independent pathways.
Billker O, Popp A, Brinkmann V, Wenig G, Schneider J, Caron E, Meyer TF
The EMBO journal 2002 Feb 15;21(4):560-71
The EMBO journal 2002 Feb 15;21(4):560-71
Homophilic adhesion of human CEACAM1 involves N-terminal domain interactions: structural analysis of the binding site.
Watt SM, Teixeira AM, Zhou GQ, Doyonnas R, Zhang Y, Grunert F, Blumberg RS, Kuroki M, Skubitz KM, Bates PA
Blood 2001 Sep 1;98(5):1469-79
Blood 2001 Sep 1;98(5):1469-79
Pathogenic Neisseria trigger expression of their carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1; previously CD66a) receptor on primary endothelial cells by activating the immediate early response transcription factor, nuclear factor-kappaB.
Muenzner P, Naumann M, Meyer TF, Gray-Owen SD
The Journal of biological chemistry 2001 Jun 29;276(26):24331-40
The Journal of biological chemistry 2001 Jun 29;276(26):24331-40
Carcinoembryonic antigen family receptor specificity of Neisseria meningitidis Opa variants influences adherence to and invasion of proinflammatory cytokine-activated endothelial cells.
Muenzner P, Dehio C, Fujiwara T, Achtman M, Meyer TF, Gray-Owen SD
Infection and immunity 2000 Jun;68(6):3601-7
Infection and immunity 2000 Jun;68(6):3601-7
Recruitment of CD55 and CD66e brush border-associated glycosylphosphatidylinositol-anchored proteins by members of the Afa/Dr diffusely adhering family of Escherichia coli that infect the human polarized intestinal Caco-2/TC7 cells.
Guignot J, Peiffer I, Bernet-Camard MF, Lublin DM, Carnoy C, Moseley SL, Servin AL
Infection and immunity 2000 Jun;68(6):3554-63
Infection and immunity 2000 Jun;68(6):3554-63
Molecular analysis of neisserial Opa protein interactions with the CEA family of receptors: identification of determinants contributing to the differential specificities of binding.
Popp A, Dehio C, Grunert F, Meyer TF, Gray-Owen SD
Cellular microbiology 1999 Sep;1(2):169-81
Cellular microbiology 1999 Sep;1(2):169-81
CEA-related proteins on human urothelial cell lines of different transformation grades.
Krop-Watorek A, Laskowska A, Salwa J, Kłopocki AG, Grunert F, Ugorski M
Cancer letters 1999 May 3;139(1):15-22
Cancer letters 1999 May 3;139(1):15-22
CD66 carcinoembryonic antigens mediate interactions between Opa-expressing Neisseria gonorrhoeae and human polymorphonuclear phagocytes.
Gray-Owen SD, Dehio C, Haude A, Grunert F, Meyer TF
The EMBO journal 1997 Jun 16;16(12):3435-45
The EMBO journal 1997 Jun 16;16(12):3435-45
Production and characterization of monoclonal antibodies against carcinoembryonic antigen (CEA).
Jantscheff P, Böttger V, Price M, Micheel B, Kaiser G, Zotter S, Kotzsch M, Grossmann H, Karsten U
Biomedica biochimica acta 1991;50(12):1261-7
Biomedica biochimica acta 1991;50(12):1261-7
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Specificity testing of D14HD11 (Prod. No. ALX-805-080). BOSC cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3, CEACAM5-CEA-CAM8 or a recombinanttransmembrane-anchored PSG1 fusion protein. Recognition of CEACAM4 was tested on CHO cells stably transfected with a CEACAM4 expression vector. Expression of the constructs was confirmed with monoclonal antibodies known to recognise the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: CAC2; CEACAM8: 80H3; PSG1: BAP1; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified D14HD11 was tested on all CEACAM transfectants. A positive signal was obtained with CEACAM1, CEACAM3, CEACAM4, CEACAM5 and CEACAM6 expressing cells (red curves).
