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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- TA804572 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA804572, RRID:AB_2627355
- Product name
- ATP5O mouse monoclonal antibody, clone OTI2E9 (formerly 2E9)
- Antibody type
- Monoclonal
- Description
- ATP5O mouse monoclonal antibody, clone OTI2E9 (formerly 2E9)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ATP5PO
- Isotype
- IgG
- Antibody clone number
- OTI2E9
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
Submitted references Stable nuclear expression of ATP8 and ATP6 genes rescues a mtDNA Complex V null mutant.
Boominathan A, Vanhoozer S, Basisty N, Powers K, Crampton AL, Wang X, Friedricks N, Schilling B, Brand MD, O'Connor MS
Nucleic acids research 2016 Nov 2;44(19):9342-9357
Nucleic acids research 2016 Nov 2;44(19):9342-9357
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP5O (RC204991, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP5O.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ATP5O mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804572)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ATP5O mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804572)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ATP5O mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804572)
- Validation comment
- IHC
