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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 66696-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATP5O Monoclonal Antibody (1D3B8)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAAPAVSGL SRQVRCFSTS VVRPFAKLVR PPVQVYGIEG RYATALYSAA SKQNKLEQVE KELLRVAQIL KEPKVAASVL NPYVKRSIKV KSLNDITAKE RFSPLTTNLI NLLAENGRLS NTQGVVSAFS TMMSVHRGEV PCTVTSASPL EEATLSELKT VLKSFLSQGQ VLKLEAKTDP SILGGMIVRI GEKYVDMSVK TKIQKLGRAM REIV (1-213 aa encoded by BC021233)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1D3B8
- Vial size
- 150 µL
- Concentration
- 0.75 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 66696-1-IG (ATP5O antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed HeLa cells using (Product # 66696-1-IG) (ATP5O antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 66696-1-IG (ATP5O antibody) at dilution of 1:300 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 66696-1-IG (ATP5O antibody) at dilution of 1:300 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
