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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PAB2131 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2131, RRID:AB_1578718
- Product name
- PI4K2A polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PI4K2A.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Phosphatidylinositol 4 phosphate regulates targeting of clathrin adaptor AP-1 complexes to the Golgi.
Cloning of a human type II phosphatidylinositol 4-kinase reveals a novel lipid kinase family.
A novel family of phosphatidylinositol 4-kinases conserved from yeast to humans.
Wang YJ, Wang J, Sun HQ, Martinez M, Sun YX, Macia E, Kirchhausen T, Albanesi JP, Roth MG, Yin HL
Cell 2003 Aug 8;114(3):299-310
Cell 2003 Aug 8;114(3):299-310
Cloning of a human type II phosphatidylinositol 4-kinase reveals a novel lipid kinase family.
Minogue S, Anderson JS, Waugh MG, dos Santos M, Corless S, Cramer R, Hsuan JJ
The Journal of biological chemistry 2001 May 18;276(20):16635-40
The Journal of biological chemistry 2001 May 18;276(20):16635-40
A novel family of phosphatidylinositol 4-kinases conserved from yeast to humans.
Barylko B, Gerber SH, Binns DD, Grichine N, Khvotchev M, Südhof TC, Albanesi JP
The Journal of biological chemistry 2001 Mar 16;276(11):7705-8
The Journal of biological chemistry 2001 Mar 16;276(11):7705-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PI4K2A polyclonal antibody (Cat # PAB2131) in HL-60 cell lysate. PI4K2A (arrow) was detected using purified Polyclonal antibody.Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- A : Negative control of HeLa cells without Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence.B : Immunofluorescence analysis of PI4K2A polyclonal antibody (Cat # PAB2131) in HeLa cells.0.025 mg/mL primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L).
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PI4K2A polyclonal antibody (Cat # PAB2131) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
