Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [10]
- Other assay [2]
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Validation data
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- Product number
- 14569-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BBS5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MSVLDALWE DRDVRFDLSA QQMKTRPGEV LIDCLDSIED TKGNNGDRGR LLVTNLRILW HSLALSRVNV SVGYNCILNI TTRTANSKLR GQTEALYILT KCNSTRFEFI FTNLVPGSPR LFTSVMAVHR AYETSKMYRD FKLRSALIQN KQLRLLPQEH VYDKINGVWN LSSDQGNLGT FFITNVRIVW HANMNDSFNV SIPYLQIRSI KIRDSKFGLA LVIESSQQSG GYVLGFKIDP VEKLQESVKE INSLHKVYSA SPIFGVDYEM EEKPQPLEAL TVEQIQDDVE IDSDGHTDAF VAYFADGNKQ QDREPVFSEE LGLAIEKLKD GFTLQGLWEV MS (1-341 aa encoded by BC044593)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.21 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human testis tissue were subjected to SDS PAGE followed by western blot with 14569-1-AP (BBS5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat testis tissue were subjected to SDS PAGE followed by western blot with 14569-1-AP (BBS5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 14569-1-AP (BBS5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF result from Dr. Corbit, Kevin. anti-BBS5 (14569-1-AP) marks the ciliary membrane and basal bodies of Human hTERT-RPE1 cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 14569-1-AP (BBS5 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 14569-1-AP (BBS5 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse kidney tissue slide using 14569-1-AP (BBS5 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse kidney tissue slide using 14569-1-AP (BBS5 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human stomach tissue slide using 14569-1-AP (BBS5 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human stomach tissue slide using 14569-1-AP (BBS5 Antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 14569-1-AP (BBS5 Antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 14569-1-AP (BBS5 Antibody) at dilution of 1:200 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 14569-1-AP ( BBS5 Antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 14569-1-AP ( BBS5 Antibody) at dilution of 1:200 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-BBS5 (IP:14569-1-AP, 3ug; Detection:14569-1-AP 1:500) with mouse testis tissue lysate 2800ug.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-BBS5 (IP:14569-1-AP, 4 ug; Detection:14569-1-AP 1:600) with mouse testis tissue lysate 4000 ug.