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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF142 - Provider product page
- Provider
- R&D Systems
- Product name
- Human DcR3/TNFRSF6B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human DcR3/TNFRSF6B in ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) TRAIL R1, rhTRAIL R2, rhTRAIL R3, and rhTRAIL R4 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O95407- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Differential effect of IL-4 and IL-13 on the expression of recombination-activating genes in mature B cells from human peripheral blood.
Kajiwara K, Shinazawa M, Morishima H, Yanagihara Y
Cellular immunology 2004 Feb;227(2):121-8
Cellular immunology 2004 Feb;227(2):121-8
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- DcR3/TNFRSF6B in Human Stomach Cancer Tissue. DcR3/TNFRSF6B was detected in immersion fixed paraffin-embedded sections of human stomach cancer using 10 µg/mL Goat Anti-Human DcR3/TNFRSF6B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF142) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the membrane of epithelial cells in gastric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- DcR3/TNFRSF6B Inhibition of Fas Ligand/TNFSF6-induced Apoptosis and Neutralization by Human DcR3/TNFRSF6B Antibody. In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (10 µg/mL, Catalog # MAB050), Recombinant Human DcR3/TNFRSF6B Fc Chimera (Catalog # 142-DC) inhibits Recombinant Human Fas Ligand/TNFSF6 (Catalog # 126-FL) induced apoptosis in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Under these conditions, inhibition of Recombinant Human Fas Ligand/TNFSF6 (2 ng/mL) activity elicited by Recombinant Human DcR3/TNFRSF6B Fc Chimera (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human DcR3/TNFRSF6B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF142). The ND50 is typically 0.1-0.5 µg/mL.
