22676-1-AP

antibody from Invitrogen Antibodies

Targeting: PPP4R2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

22676-1-AP

Provider

Invitrogen Antibodies

Product name

PPP4R2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MDVERLQEA LKDFEKRGKK EVCPVLDQFL CHVAKTGETI IPFTIQRLCE LLTDPRRNYT GTDKFLRGVE KNVMVVSCVY PSSEKNNSNS LNRMNGVMFP GNSPSYTERS NINGPGTPRP LNRPKVSLSA PMTTNGLPES TDSKEANLQQ NEEKNHSDSS TSESEVSSVS PLKNKHPDED AVEAEGHEVK RLRFDKEGEV RETASQ (1-205 aa encoded by B C110889)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.37 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 22676-1-AP (PPP4R2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 22676-1-AP (PPP4R2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 22676-1-AP (PPP4R2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 22676-1-AP (PPP4R2 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 22676-1-AP (PPP4R2 Antibody) at dilution of 1:50 (under 25x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 22676-1-AP ( PPP4R2 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 22676-1-AP ( PPP4R2 Antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PPP4R2 (IP:22676-1-AP, 4ug; Detection:22676-1-AP 1:1000) with HepG2 cells lysate 3600ug.