Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-2877 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2877, RRID:AB_2237475
- Product name
- Rabbit Polyclonal PPP4R2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references PPP4R2 regulates neuronal cell differentiation and survival, functionally cooperating with SMN.
A PP2A phosphatase high density interaction network identifies a novel striatin-interacting phosphatase and kinase complex linked to the cerebral cavernous malformation 3 (CCM3) protein.
Bosio Y, Berto G, Camera P, Bianchi F, Ambrogio C, Claus P, Di Cunto F
European journal of cell biology 2012 Aug;91(8):662-74
European journal of cell biology 2012 Aug;91(8):662-74
A PP2A phosphatase high density interaction network identifies a novel striatin-interacting phosphatase and kinase complex linked to the cerebral cavernous malformation 3 (CCM3) protein.
Goudreault M, D'Ambrosio LM, Kean MJ, Mullin MJ, Larsen BG, Sanchez A, Chaudhry S, Chen GI, Sicheri F, Nesvizhskii AI, Aebersold R, Raught B, Gingras AC
Molecular & cellular proteomics : MCP 2009 Jan;8(1):157-71
Molecular & cellular proteomics : MCP 2009 Jan;8(1):157-71
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PPP4R2 Antibody [NB100-2877] - Detection of Human and Mouse PPP4R2 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PPP4R2 antibody NB100-2877 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PPP4R2 Antibody [NB100-2877] - Detection of Human and Mouse PPP4R2 on HeLa whole cell lysate using NB100-2877.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PPP4R2 Antibody [NB100-2877] - Detection of human PPP4R2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-PPP4R2 antibody NB100-2877 (lotNB100-2877-2) used for IP at 6 ug per reaction. PPP4R2 was also immunoprecipitated by a previous lot of this antibody (lot NB100-2877-1). For blotting immunoprecipitated PPP4R2,NB100-2877 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PPP4R2 Antibody [NB100-2877] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PPP4R2 used at a dilution of 1:200 (1ug/ml). Detection: DAB